Wound-induced p38MAPK-dependent histone H3 phosphorylation correlates with increased COX-2 expression in enterocytes

Journal of Cellular Physiology
Thomas KarraschChristian Jobin

Abstract

Gastrointestinal epithelial cell damage triggers an important biological response called restitution, a process aimed at re-epithelializing the wounded areas. Unfortunately, little is known about the intrinsic molecular signaling events implicated in this host response. We hypothesized that wounding intestinal epithelial cells activates signaling pathways leading to chromatin modification and COX-2 upregulation during restitution. Confluent rat IEC18 cells were mechanically wounded by multiple parallel scratches using a pipet tip. NF-kappaB(Ser536), p38, and histone H3(Ser10) (H3S10) phosphorylation were determined by Western blot using specific phospho-antibodies. COX-2 gene expression was evaluated by RT-PCR, Western Blot, and ELISA. Association of phosphorylated H3, RelA (NF-kappaB), and RNA polymerase II to the COX-2 gene promoter was evaluated by chromatin immunoprecipitation (ChIP). The specific inhibitors Bay11-7082 and SB239063 as well as Ad5IkappaB-superrepressor (Ad5IkappaBAA) and Ad5dnp38 were used to block NF-kappaB- and p38-signaling pathways, respectively. Wounding induced a rapid and sustained (24 h) phosphorylation of RelAS536, H3S10, and p38MAPK in enterocytes. ChIP analysis of the COX-2 gene promoter demonstra...Continue Reading

References

Mar 1, 1978·European Journal of Biochemistry·L R GurleyR A Tobey
Mar 5, 1996·Proceedings of the National Academy of Sciences of the United States of America·R J PlayfordA M Hanby
Apr 17, 1997·Biochemical and Biophysical Research Communications·B K DieckgraefeD H Alpers
Feb 23, 2000·The Journal of Clinical Investigation·O MorteauR B Sartor
May 29, 2000·The Journal of Biological Chemistry·H InoueK Umesono
Mar 10, 2001·Inflammatory Bowel Diseases·A U Dignass
Apr 26, 2001·The Journal of Surgical Research·E D StrauchB L Bass
Aug 11, 2001·Science·T Jenuwein, C D Allis
Dec 18, 2001·Nature Immunology·Simona SaccaniGioacchino Natoli
Mar 13, 2002·Gut·V Arulampalam, S Pettersson
Jun 27, 2003·American Journal of Physiology. Cell Physiology·Laurence J EganMartin F Kagnoff
Aug 9, 2003·Critical Care Medicine·Joshua M V Mammen, Jeffrey B Matthews
Dec 16, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Gye Young ParkJohn W Christman
May 1, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Timothy D Warner, Jane A Mitchell
Jul 23, 2004·Current Biology : CB·Craig L Peterson, Marc-André Laniel
Aug 4, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Eike HollenbachMichael Naumann
Sep 1, 2004·Cellular and Molecular Life Sciences : CMLS·A Verger, M Crossley
Feb 17, 2005·The Journal of Pathology·R J Playford, S Ghosh

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Citations

Sep 1, 2007·Inflammatory Bowel Diseases·Thomas Karrasch, Christian Jobin
Feb 2, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Jakob Benedict SeidelinOle Haagen Nielsen
Apr 18, 2007·Journal of Clinical Gastroenterology·R Balfour Sartor
Jan 13, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kristen L W WaltonR Balfour Sartor
Jan 31, 2009·American Journal of Physiology. Gastrointestinal and Liver Physiology·Christopher P GayerMarc D Basson
Oct 16, 2008·World Journal of Gastroenterology : WJG·Takashi NishimuraYoshihide Fujiyama
Feb 4, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Joshua TercSimon A Hirota
Mar 13, 2012·Journal of Crohn's & Colitis·Florian RiederMiquel Sans
Dec 28, 2010·Clinica Chimica Acta; International Journal of Clinical Chemistry·Mehmet CoskunOle Haagen Nielsen
Aug 11, 2007·Molecular and Cellular Endocrinology·Olivier Kassel, Peter Herrlich
Jun 6, 2014·Diabetes/metabolism Research and Reviews·Wei-Jian NiWei Wei
Aug 2, 2011·The American Journal of Pathology·Jason R GoldsmithChristian Jobin
Feb 20, 2015·Journal of Cancer Research and Therapeutics·Wen-Yan YangTian-Min Zhen
Oct 27, 2015·The FEBS Journal·Maria Eugenia DelgadoThomas Brunner
Feb 1, 2017·BMC Pulmonary Medicine·Dorota StefanowiczTillie-Louise Hackett
Jan 19, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kaitlin M NauglerMark J Ropeleski
May 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Thomas KarraschChristian Jobin
Feb 6, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Kris A SteinbrecherAlbert S Baldwin
Nov 15, 2014·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jakob Benedict SeidelinOle Haagen Nielsen
Oct 2, 2007·Current Opinion in Gastroenterology·Declan F McCole, Kim E Barrett
Jul 1, 2020·Terapevticheskiĭ arkhiv·M Y ZvyaglovaA I Parfenov

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