Wss1 Promotes Replication Stress Tolerance by Degrading Histones.

Cell Reports
Karthik MaddiIvan Dikic

Abstract

Timely completion of DNA replication is central to accurate cell division and to the maintenance of genomic stability. However, certain DNA-protein interactions can physically impede DNA replication fork progression. Cells remove or bypass these physical impediments by different mechanisms to preserve DNA macromolecule integrity and genome stability. In Saccharomyces cerevisiae, Wss1, the DNA-protein crosslink repair protease, allows cells to tolerate hydroxyurea-induced replication stress, but the underlying mechanism by which Wss1 promotes this function has remained unknown. Here, we report that Wss1 provides cells tolerance to replication stress by directly degrading core histone subunits that non-specifically and non-covalently bind to single-stranded DNA. Unlike Wss1-dependent proteolysis of covalent DNA-protein crosslinks, proteolysis of histones does not require Cdc48 nor SUMO-binding activities. Wss1 thus acts as a multi-functional protease capable of targeting a broad range of covalent and non-covalent DNA-binding proteins to preserve genome stability during adverse conditions.

Citations

Jul 22, 2020·Seminars in Cell & Developmental Biology·Juliette FerrandSophie E Polo
Nov 4, 2020·Current Genetics·Aimorn HomchanDanaya Pakotiprapha
Jan 6, 2021·Communications Biology·Annamaria Ruggiano, Kristijan Ramadan
Jan 8, 2021·Frontiers in Cell and Developmental Biology·Manideep C PachvaRegina Groisman

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