DOI: 10.1101/468421Nov 12, 2018Paper

WUSCHEL acts as a rheostat on the auxin pathway to maintain apical stem cells in Arabidopsis

BioRxiv : the Preprint Server for Biology
Yanfei MaJan Lohmann

Abstract

To maintain the balance between long-term stem cell self-renewal and differentiation, dynamic signals need to be translated into spatially precise and temporally stable gene expression states. In the apical plant stem cell system, local accumulation of the small, highly mobile phytohormone auxin triggers differentiation while at the same time, pluripotent stem cells are maintained throughout the entire life-cycle. We find that stem cells are resistant to auxin mediated differentiation, but require low levels of signaling for their maintenance. We demonstrate that the WUSCHEL transcription factor confers this behavior by rheostatically controlling the auxin signaling and response pathway. Finally, we show that WUSCHEL acts via regulation of histone acetylation at target loci, including those with functions in the auxin pathway. Our results reveal an important mechanism that allows cells to differentially translate a potent and highly dynamic developmental signal into stable cell behavior with high spatial precision and temporal robustness.

Related Concepts

Auxins
Cell Differentiation Process
Gene Expression
Spatial Distribution
Stem Cells
Transcription Factor
Arabidopsis thaliana <plant>
Apical
Genetic Loci
Pluripotent Stem Cells

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