WW domain-binding protein 2 acts as an oncogene by modulating the activity of the glycolytic enzyme ENO1 in glioma

Cell Death & Disease
Shuai ChenChi-Meng Tzeng

Abstract

WW domain-binding protein 2 (WBP2) has been demonstrated as oncogenic in breast cancer. Many studies have revealed the WBP2 gene as a high-risk gene for leukoariaosis and cerebral white matter lesions is important in the pathologic stage of glioma development. This study aimed to illustrate the underlying mechanism by which WBP2 regulates the process of glioma development. The expression pattern of WBP2 in several tumor cells was determined, clarifying the carcinogenic action of WBP2 in glioma cells. Overexpression of WBP2 in glioma cells promoted cell proliferation and migration, and the number of S-phase cells, whereas the depletion of WBP2 by RNAi-mediated knockdown restrained cell growth and cell cycle progression. Upregulation of WBP2 significantly enhanced the tumorigenic ability of U251 cells in vivo. MS/GST pulldown assay identified α-enolase (ENO1) and Homer protein homolog 3 (Homer3) as novel potent interaction partners of WBP2. Knockdown of ENO1 or Homer3 allowed cell growth and migration to return to normal levels. Furthermore, in vitro and in vivo experiments indicated that the oncogenic role of WBP2 in glioma was through modulating ENO1 and glycolysis activity via the ENO1-PI3K/Akt signaling pathway. Collectively,...Continue Reading

References

Mar 1, 1990·The American Journal of Physiology·R S Balaban
Aug 15, 1995·Proceedings of the National Academy of Sciences of the United States of America·H I Chen, M Sudol
Aug 11, 2001·Cellular and Molecular Life Sciences : CMLS·V Pancholi
Mar 26, 2002·FEBS Letters·Maria J MaciasMarius Sudol
Oct 9, 2003·The Biochemical Journal·Roberto NitschMariastella Zannini
Apr 30, 2004·The Journal of Comparative Neurology·Yoko ShiraishiTeiichi Furuichi
Aug 24, 2006·Biochimica Et Biophysica Acta·Hideki Yamaguchi, John Condeelis
Feb 24, 2007·Genome Biology·Yoko Shiraishi-Yamaguchi, Teiichi Furuichi
Sep 15, 2007·Molecular & Cellular Proteomics : MCP·Yunhao ChenYoon-Pin Lim
Oct 25, 2007·Cellular and Molecular Life Sciences : CMLS·E A McSherryS McDonnell
Dec 3, 2008·Journal of Neurochemistry·Lisa Foa, Robert Gasperini
Jan 25, 2011·Nature Reviews. Cancer·Rob A CairnsTak W Mak
Jun 7, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Shen Kiat LimYoon Pin Lim
Jun 15, 2011·Advanced Drug Delivery Reviews·Trenis D PalmerAndries Zijlstra
Jun 18, 2011·Annals of Neurology·Myriam FornageLenore J Launer
Oct 12, 2011·Annual Review of Cell and Developmental Biology·Sophia Y Lunt, Matthew G Vander Heiden
Feb 15, 2013·The Journal of Biological Chemistry·Ethan PoteetShao-Hua Yang
Jan 28, 2014·American Journal of Human Genetics·Kathleen A WilliamsonDavid R Fitzpatrick
Jul 30, 2014·Cell Reports·Paolo E PorporatoPierre Sonveaux
Jul 15, 2015·Biomarkers in Cancer·Seyedmehdi NourashrafeddinMohammad Hossein Modarressi
Feb 18, 2016·EMBO Molecular Medicine·Annalisa BunielloKaren P Steel
Jul 18, 2016·Journal of the Neurological Sciences·Guo-Zhong YiSong-Tao Qi
Mar 21, 2017·Trends in Endocrinology and Metabolism : TEM·Galina GabrielyFrancisco J Quintana

❮ Previous
Next ❯

Citations

May 13, 2020·Oncogene·Hossein TabatabaeianYoon Pin Lim
Aug 9, 2020·OncoTargets and Therapy·Wei HanWei Guan
Aug 11, 2020·Journal for Immunotherapy of Cancer·Robyn A A OldhamJeffrey A Medin
Jan 21, 2020·Journal of Cellular and Molecular Medicine·Rui ChenShun Fang
Sep 8, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jianxiong JiJian Wang
Jan 22, 2021·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Melissa HumYoon Pin Lim
Jul 9, 2021·Drug Design, Development and Therapy·Xiaotao SuChao Qin

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry
pulldown
transfection
chip
coimmunoprecipitation
PCR
Assay

Software Mentioned

GraphPad Prism
Oncomine

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

AKT Pathway

This feed focuses on the AKT serine/threonine kinase, which is an important signaling pathway involved in processes such as glucose metabolism and cell survival.