WWC3 inhibits epithelial-mesenchymal transition of lung cancer by activating Hippo-YAP signaling

OncoTargets and Therapy
Qiang HanEnhua Wang

Abstract

Though we recently reported that the WWC3 inhibits the invasiveness and metastasis of lung cancer by activating the Hippo pathway, the impact and underlying mechanisms of this process still remain unclear. To identify the role of WWC3 in epithelial-mesenchymal transition of lung cancer, we performed immunohistochemistry to detect the expression levels of WWC3 and EMT-related biomarker, and analyzed their correlations in a cohort of 127 patients with NSCLC. Wound healing assay and cell invasion assay were applied to explore cell invasive ability change after WWC3 knockdown. qRT-PCR and immunoblotting were performed to assess mRNA and protein levels of EMT-related biomarkers and the main molecules changes of Hippo signaling caused by WWC3. Immunoprecipition was to examine WWC3 and LATS1 interaction. WWC3 knockdown drives a pronounced shift from the epithelial to the mesenchymal phenotype in lung cancer cells. In addition, WWC3 ectopic expression in lung cancer cells attenuates mesenchymal markers and increases the epithelial markers expressions; however, WWC3-ΔWW plasmid abrogated these effects. WWC3 silencing by shRNA exerts the opposite effect. Furthermore, WWC3 levels were inversely correlated with the levels of EMT inducers (...Continue Reading

Citations

Jul 26, 2018·Oncotarget·Ashley L MussellJianmin Zhang
Oct 13, 2020·Frontiers in Cell and Developmental Biology·Giorgio VirnicchiAlexander W Bruce
Jan 21, 2021·Cancers·Verena HöffkenJoachim Kremerskothen
Oct 30, 2020·OncoTargets and Therapy·Mu ChenChunlong Zhong

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Methods Mentioned

BETA
surgical resection
transfection
electrophoresis
confocal

Software Mentioned

SPSS
ImageJ
Cell Counter

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