Oct 4, 2013

wzi Gene sequencing, a rapid method for determination of capsular type for Klebsiella strains

Journal of Clinical Microbiology
Sylvain BrisseDominique Decré

Abstract

Pathogens of the genus Klebsiella have been classified into distinct capsular (K) types for nearly a century. K typing of Klebsiella species still has important applications in epidemiology and clinical microbiology, but the serological method has strong practical limitations. Our objective was to evaluate the sequencing of wzi, a gene conserved in all capsular types of Klebsiella pneumoniae that codes for an outer membrane protein involved in capsule attachment to the cell surface, as a simple and rapid method for the prediction of K type. The sequencing of a 447-nucleotide region of wzi distinguished the K-type reference strains with only nine exceptions. A reference wzi sequence database was created by the inclusion of multiple strains representing K types associated with high virulence and multidrug resistance. A collection of 119 prospective clinical isolates of K. pneumoniae were then analyzed in parallel by wzi sequencing and classical K typing. Whereas K typing achieved typeability for 81% and discrimination for 94.4% of the isolates, these figures were 98.1% and 98.3%, respectively, for wzi sequencing. The prediction of K type once the wzi allele was known was 94%. wzi sequencing is a rapid and simple method for the de...Continue Reading

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  • Citations57

Citations

Mentioned in this Paper

Microbial Anatomical Capsule Structure
Clinical Microbiology
Klebsiella pneumoniae
Nucleic Acid Sequencing
Outer Membrane Lipoproteins, Bacterial
Rapid Technique
Infection by Klebsiella Pneumoniae in Conditions Classified Elsewhere and of Unspecified Site
Sequencing
Genes, vif
Sequence Determinations, DNA

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