X-gene product of hepatitis B virus induces apoptosis in liver cells.

The Journal of Biological Chemistry
H KimY Yun

Abstract

Hepatitis B virus is a causative agent of hepatocellular carcinoma, and in the course of tumorigenesis, the X-gene product (HBx) is known to play important roles. Here, we investigated the transforming potential of HBx by conventional focus formation assay in NIH3T3 cells. Cells were cotransfected with the HBx expression plasmid along with other oncogenes including Ha-ras, v-src, v-myc, v-fos, and E1a. Unexpectedly, the introduction of HBx completely abrogated the focus-forming ability of all five tested oncogenes. In addition, the cotransfection of Bcl-2, an apoptosis inhibitor, reversed the HBx-mediated inhibition of focus formation, suggesting that the observed repression of focus formation by HBx is through the induction of apoptosis. Next, to test unequivocally whether HBx induces apoptosis in liver cells, we established stable Chang liver cell lines expressing HBx under the control of a tetracycline-inducible promoter. Induction of HBx in these cells in the presence of 1% calf serum resulted in typical apoptosis phenomena such as DNA fragmentation, nuclear condensation, and fragmentation. Based on these results, we propose that HBx sensitizes liver cells to apoptosis upon hepatitis B virus infection, contributing to the d...Continue Reading

References

Jan 1, 1992·Journal of Virological Methods·D K HoweG L McLaughlin
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·L RaoE White
Jun 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·F A OberhammerR Schulte-Hermann
Jul 1, 1989·Japanese Journal of Cancer Research : Gann·Y ShirakataK Koike
Jan 1, 1987·Annual Review of Biochemistry·D Ganem, H E Varmus
Nov 21, 1995·Proceedings of the National Academy of Sciences of the United States of America·J Benn, R J Schneider
Nov 24, 1995·Science·Z XiaM E Greenberg
Dec 1, 1995·Journal of Virology·H ZhuT Shenk
Nov 1, 1994·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·W BurschR Schulte-Hermann
Feb 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·A PuisieuxM Ozturk
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·J Benn, R J Schneider
Mar 1, 1994·Journal of Virology·F ZoulimC Seeger
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·X W WangC C Harris
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·S A McCarthyT Van Dyke
Apr 1, 1993·Genes & Development·M Debbas, E White
Mar 1, 1997·Journal of Virology·J G Teodoro, P E Branton
Mar 6, 1997·Biochemical and Biophysical Research Communications·A CapovillaP Arbuthnot
Aug 1, 1997·Journal of Hepatology·P R Galle

❮ Previous
Next ❯

Citations

Sep 25, 1999·Journal of Cellular Physiology·M A Feitelson
Nov 9, 2001·Biochemical and Biophysical Research Communications·M O LeeS J Kim
Oct 31, 2007·Child's Nervous System : ChNS : Official Journal of the International Society for Pediatric Neurosurgery·Seok-Gu KangChun Kun Park
Jun 13, 2009·Apoptosis : an International Journal on Programmed Cell Death·Chi-Hung LinHung-Jeng Liu
Nov 2, 2012·Cancer Metastasis Reviews·Mausumi BharadwajShowket Hussain
May 15, 2007·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Lu YeShihui Song
Apr 16, 2004·Virus Research·Jing-Chyi WangGuang-Yuh Hwang
Sep 5, 2002·Biochemical and Biophysical Research Communications·Chawon YunHyeseong Cho
Apr 29, 2000·Biochimica Et Biophysica Acta·M J KosovskyB Yoffe
Apr 11, 2003·Virus Research·Milena PantevaShahid Jameel
Sep 2, 1998·Current Opinion in Immunology·M Barry, G McFadden
Apr 18, 2000·Mutation Research·S P Hussain, C C Harris
Aug 27, 2005·Mitochondrion·Kyung-Won Huh, Aleem Siddiqui
Jul 17, 2001·International Journal of Experimental Pathology·P Arbuthnot, M Kew
Oct 5, 2001·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·J LarkinM A Feitelson
Oct 7, 2006·Current Opinion in Gastroenterology·G A Macdonald
Jan 14, 2011·Journal of Gastroenterology and Hepatology·Michael C Kew
Nov 16, 2004·Journal of Virology·Michael J Bouchard, Robert J Schneider
Mar 8, 2000·Microbiology and Molecular Biology Reviews : MMBR·C Seeger, W S Mason
Jul 1, 2006·Biomedical Imaging and Intervention Journal·Ch Wong, Kl Goh
Dec 4, 2012·Viruses·Siddhartha RawatMichael J Bouchard
Apr 18, 2012·World Journal of Gastroenterology : WJG·Ren-Xian TangKui-Yang Zheng
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·J Guilherme TralhaoChristian Bréchot
Feb 10, 2016·Proceedings of the National Academy of Sciences of the United States of America·Tianyu JiangYigong Shi
Dec 19, 2012·Medicina clínica·Samuel Martín-VílchezUNKNOWN Grupo CIBERehd de Hepatología del Hospital Universitario de La Princesa

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

BCL-2 Family Proteins

BLC-2 family proteins are a group that share the same homologous BH domain. They play many different roles including pro-survival signals, mitochondria-mediated apoptosis and removal or damaged cells. They are often regulated by phosphorylation, affecting their catalytic activity. Here is the latest research on BCL-2 family proteins.

Carcinoma, Hepatocellular

Hepatocellular Carcinoma is a malignant cancer in liver epithelial cells. Discover the latest research on Hepatocellular Carcinoma here.

Adrenocortical Carcinoma

Hepatocellular carcinoma is the most common type of primary liver cancer and frequently occurs in individuals with chronic liver diseases like cirrhosis. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
P ChirilloM Levrero
Proceedings of the National Academy of Sciences of the United States of America
F Su, Robert J Schneider
Proceedings of the National Academy of Sciences of the United States of America
J Benn, Robert J Schneider
Proceedings of the National Academy of Sciences of the United States of America
L W ElmoreC C Harris
© 2021 Meta ULC. All rights reserved