X-ray crystal structure of the rotavirus inner capsid particle at 3.8 A resolution.

Journal of Molecular Biology
Brian McClainS C Harrison

Abstract

The rotavirus inner capsid particle, known as the "double-layered particle" (DLP), is the "payload" delivered into a cell in the process of viral infection. Its inner and outer protein layers, composed of viral protein (VP) 2 and VP6, respectively, package the 11 segments of the double-stranded RNA (dsRNA) of the viral genome, as well as about the same number of polymerase molecules (VP1) and capping-enzyme molecules (VP3). We have determined the crystal structure of the bovine rotavirus DLP. There is one full particle (outer diameter approximately 700 A) in the asymmetric unit of the P2(1)2(1)2(1) unit cell of dimensions a=740 A, b=1198 A, and c=1345 A. A three-dimensional reconstruction from electron cryomicroscopy was used as a molecular replacement model for initial phase determination to about 18.5 A resolution, and the 60-fold redundancy of icosahedral particle symmetry allowed phases to be extended stepwise to the limiting resolution of the data (3.8 A). The structure of a VP6 trimer (determined previously by others) fits the outer layer density with very little adjustment. The T=13 triangulation number of that layer implies that there are four and one-third VP6 trimers per icosahedral asymmetric unit. The inner layer ha...Continue Reading

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Citations

Jan 24, 2012·Nature Reviews. Microbiology·Shane D TraskJohn T Patton
Jan 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lingpeng ChengPing Zhu
Apr 12, 2012·Proceedings of the National Academy of Sciences of the United States of America·Chongwen YangLingpeng Cheng
Jan 21, 2011·Journal of Virology·Sarah M McDonald, John T Patton
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Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Javier PerizAchillefs N Kapanidis
Nov 21, 2013·Virologica Sinica·Xiaoming Li, Qin Fang
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May 19, 2012·Current Opinion in Virology·Shane D TraskJohn T Patton
Dec 16, 2010·The EMBO Journal·Ethan C SettembreStephen C Harrison
Apr 19, 2013·The Journal of General Virology·Crystal E BoudreauxSarah M McDonald
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Oct 24, 2012·Journal of Molecular Biology·Leandro F EstroziStephen C Harrison
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Sep 13, 2018·Viruses·Daniel LuqueJosé R Castón

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