X-ray crystallographic structure of the Norwalk virus protease at 1.5-A resolution.

Journal of Virology
Corinne E ZeitlerB V Venkataram Prasad

Abstract

Norwalk virus (NV), a member of the Caliciviridae family, is the major cause of acute, epidemic, viral gastroenteritis. The NV genome is a positive sense, single-stranded RNA that encodes three open reading frames (ORFs). The first ORF produces a polyprotein that is processed by the viral cysteine protease into six nonstructural proteins. We have determined the structure of the NV protease to 1.5 and 2.2 A from crystals grown in the absence or presence, respectively, of the protease inhibitor AEBSF [4-(2-aminoethyl)-benzenesulfonyl fluoride]. The protease, which crystallizes as a stable dimer, exhibits a two-domain structure similar to those of other viral cysteine proteases with a catalytic triad composed of His 30, Glu 54, and Cys 139. The native structure of the protease reveals strong hydrogen bond interactions between His 30 and Glu 54, in the favorable syn configuration, indicating a role of Glu 54 during proteolysis. Mutation of this residue to Ala abolished the protease activity, in a fluorogenic peptide substrate assay, further substantiating the role of Glu 54 during proteolysis. These observations contrast with the suggestion, from a previous study of another norovirus protease, that this residue may not have a promi...Continue Reading

References

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·J F Bazan, R J Fletterick
Jun 14, 1994·Biochemistry·N H YennawarG K Farber
Jun 20, 1993·Journal of Molecular Biology·R A LaskowskiJ M Thornton
Jan 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·S Jones, J M Thornton
Jan 7, 1998·Journal of Molecular Biology·S C MosimannM N James
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·D A MatthewsS T Worland
Oct 3, 1999·Virus Research·J SeipeltT Skern
Oct 26, 1999·Acta Crystallographica. Section D, Biological Crystallography·J W Pflugrath
Jun 23, 2000·Journal of Virology·P J GlassM K Estes
Sep 22, 2001·Acta Crystallographica. Section D, Biological Crystallography·A PerrakisV S Lamzin
Feb 5, 1997·Chemical Reviews·Hans-Hartwig Otto, Tanja Schirmeister
May 22, 2002·Journal of Virology·Yuichi SomeyaTatsuo Miyamura
Oct 9, 2002·Virus Research·Michele E HardyKhalil Ettayebi
Apr 23, 2003·Virology·Susan J BlakeneyPatricia A Reilly
Oct 24, 2003·Acta Crystallographica. Section D, Biological Crystallography·G BricogneW Paciorek
Feb 7, 2004·The Journal of Biological Chemistry·Kenneth K-S NgFrancisco Parra
May 1, 1997·Acta Crystallographica. Section D, Biological Crystallography·G N MurshudovE J Dodson
Sep 14, 2004·The Biochemical Journal·Hannelore PetersVerena Gauss-Müller
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Apr 5, 2005·Acta Crystallographica. Section D, Biological Crystallography·Airlie J McCoyRandy J Read
Apr 23, 2005·Virus Research·Yuichi SomeyaTatsuo Miyamura
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

Jun 8, 2011·Biomolecular NMR Assignments·Daisuke TakahashiOm Prakash
Jun 27, 2009·Journal of Biochemistry·Yuichi Someya, Naokazu Takeda
Nov 4, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·R J HusseyP M Shoolingin-Jordan
Oct 5, 2012·Clinical and Vaccine Immunology : CVI·Nadim J AjamiMary K Estes
Aug 24, 2012·Journal of Virology·Lucy ThorneIan Goodfellow
Nov 24, 2006·Journal of Virology·Stephen W B FullertonJacques Rohayem
Feb 1, 2013·Journal of Virology·Zana MuhaxhiriB V Venkataram Prasad
Sep 21, 2010·Uirusu·Hironori Sato
Jun 4, 2014·Biochemical Pharmacology·Joana Rocha-PereiraDirk Jochmans
Feb 1, 2012·Biomolecular Concepts·Yuichi Someya
Aug 11, 2015·Journal of Medicinal Chemistry·Yunjeong KimWilliam C Groutas
Sep 7, 2013·Journal of Medical Virology·Chi-Neu TsaiShih-Yen Chen
Mar 10, 2016·Annual Review of Virology·Stephanie M Karst, Christiane E Wobus
Oct 8, 2008·Immunological Reviews·Eric F DonaldsonRalph S Baric
Nov 19, 2013·The Journal of General Virology·Lucy G Thorne, Ian G Goodfellow
Apr 6, 2016·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Soroush T SarvestaniJason M Mackenzie
Sep 24, 2013·Virus Research·Daisuke TakahashiKyeong-Ok Chang
Nov 13, 2012·Virology·Chao WeiXi Jiang
Oct 16, 2012·Biochemical and Biophysical Research Communications·J Rocha-PereiraJ Neyts
Feb 13, 2013·Virology·Prasanth ViswanathanBrent Korba
Apr 26, 2016·Expert Opinion on Drug Discovery·Sahani WeerasekaraDuy H Hua
Jun 20, 2016·Current Opinion in Virology·Bv Venkataram PrasadRobert L Atmar
Nov 9, 2017·Viruses·Ji-Hye LeeKyung Hyun Kim
Mar 14, 2018·Antimicrobial Agents and Chemotherapy·Mitsutaka KitanoIan Goodfellow
Jan 16, 2013·Protein Science : a Publication of the Protein Society·Daisuke TakahashiOm Prakash
Aug 31, 2006·Current Opinion in Infectious Diseases·Mary K EstesRobert L Atmar
Jan 11, 2019·Journal of Virology·Mariya A ViskovskaB V Venkataram Prasad
May 22, 2007·Biochemical Society Transactions·S CurryR J Leatherbarrow
Dec 21, 2010·FEBS Letters·Yuichi Someya, Naokazu Takeda
Jan 6, 2011·Microbiology and Immunology·Tomoichiro OkaKazuhiko Katayama
Feb 18, 2016·Expert Opinion on Therapeutic Patents·Anushka C Galasiti KankanamalageWilliam C Groutas
Jul 3, 2020·Frontiers in Immunology·Claudia P Campillay-VélizMargarita K Lay

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