X-ray diffraction analysis and molecular-replacement solution of the cyan fluorescent protein dsFP483

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Meitian WangRebekka M Wachter

Abstract

A novel cyan fluorescent protein, dsFP483 from the coral Discosoma striata, has been crystallized. Diffraction data were collected to 2.1 A and processed in space group C2. Molecular-replacement methods were applied using the closely related red fluorescent protein DsRed as a search model. The asymmetric unit appears to contain six protein molecules (1.5 tetramers), five of which (83%) could be located by the molecular-replacement searches.

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Citations

Apr 9, 2008·Journal of Molecular Biology·Gabrielle D MaloRebekka M Wachter

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