X-ray fluorescence detection for serial macromolecular crystallography using a JUNGFRAU pixel detector

Journal of Synchrotron Radiation
Isabelle MartielMeitian Wang

Abstract

Detection of heavy elements, such as metals, in macromolecular crystallography (MX) samples by X-ray fluorescence is a function traditionally covered at synchrotron MX beamlines by silicon drift detectors, which cannot be used at X-ray free-electron lasers because of the very short duration of the X-ray pulses. Here it is shown that the hybrid pixel charge-integrating detector JUNGFRAU can fulfill this function when operating in a low-flux regime. The feasibility of precise position determination of micrometre-sized metal marks is also demonstrated, to be used as fiducials for offline prelocation in serial crystallography experiments, based on the specific fluorescence signal measured with JUNGFRAU, both at the synchrotron and at SwissFEL. Finally, the measurement of elemental absorption edges at a synchrotron beamline using JUNGFRAU is also demonstrated.

References

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Citations

Sep 4, 2021·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Nicholas J TerrillWim Bras

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Methods Mentioned

BETA
X-ray
chip
chips
Fluorescence

Software Mentioned

EIGER
PILATUS
JUNGFRAU

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