PMID: 9448294Mar 14, 1998Paper

X-ray-induced mutations in mouse embryonic stem cells

Proceedings of the National Academy of Sciences of the United States of America
J W ThomasT Magnuson

Abstract

Deletion complexes consisting of multiple chromosomal deletions induced at single loci can provide a means for functional analysis of regions spanning several centimorgans in model genetic systems. A strategy to identify and map deletions at any cloned locus in the mouse is described here. First, a highly polymorphic, germ-line competent F1(129/Sv-+Tyr+p x CAST/Ei) mouse embryonic stem cell line was established. Then, x-ray and UV-induced mutagenesis was performed to determine the feasibility of generating deletion complexes throughout the mouse genome. Reported here are the selection protocols, induced mutation frequencies, cytogenetic and extensive molecular analysis of mutations at the X-chromosome-linked hypoxanthine phosphoribosyltransferase (Hprt) locus and at the neural cell adhesion molecule (Ncam) locus located on chromosome 9. Mutation analysis with PCR-based polymorphic microsatellite markers revealed deletions of <3 cM at the Hprt locus, whereas results consistent with deletions covering >28 cM were observed at the Ncam locus. Fluorescence in situ hybridization with a chromosome 9 paint revealed that some of the Ncam deletions were accompanied by complex chromosome rearrangements. In addition, deletion mapping in co...Continue Reading

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·W L RussellE L Phipps
Jan 1, 1979·Cell·C T Caskey, G D Kruh
Jan 1, 1992·Somatic Cell and Molecular Genetics·C Y LiJ B Little
Mar 1, 1992·Analytical Biochemistry·R Ramírez-SolisA Bradley
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·T YagiS Aizawa
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E M RinchikP B Selby
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·B H KollerO Smithies
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H H EvansJ Hozier
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·T DoetschmanO Smithies
Mar 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·S Gluecksohn-WaelschC F Cori
Feb 1, 1966·Genetical Research·M F Lyon, T Morris
Jul 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·P KavathasR DeMars
Jun 1, 1994·Nature Genetics·W F DietrichR D Dredge
Jan 1, 1994·Somatic Cell and Molecular Genetics·J C FuscoeG Pilia
Jan 1, 1993·Mammalian Genome : Official Journal of the International Mammalian Genome Society·E M Rinchik, D A Carpenter
Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T Morris, J Thacker
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·J E RabinowitzT Magnuson
Jan 1, 1951·Cold Spring Harbor Symposia on Quantitative Biology·W L RUSSELL

❮ Previous
Next ❯

Citations

Oct 6, 2000·Genesis : the Journal of Genetics and Development·Y T Chen, A Bradley
Jan 13, 2004·Neurotoxicity Research·Mary B NewmanPaul R Sanberg
Oct 26, 2005·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Wallace S H ChickYun You
Feb 23, 2011·Cancer Metastasis Reviews·Lesley A MathewsWilliam L Farrar
Aug 1, 1998·Mutation Research·R P WoychikE D Avrer
Jun 14, 2005·Nature Reviews. Genetics·Benjamin T Kile, Douglas J Hilton
Dec 28, 1999·Molecular and Cellular Biology·B ZhengA Bradley
Mar 26, 2005·Journal of Clinical Pathology·M R HusseinG S Wood
Nov 10, 2001·Annual Review of Genomics and Human Genetics·R Balling
Jul 11, 2006·Annual Review of Genomics and Human Genetics·Louise van der Weyden, Allan Bradley
Jan 9, 2014·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·S LuftS Ritter
Sep 14, 2000·Proceedings of the National Academy of Sciences of the United States of America·D F LePageR A Conlon
Nov 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Aubrey C FrankTimothy P O'Brien
Dec 18, 2003·The Journal of Physiology·Timothy P O'Brien, Wayne N Frankel
Oct 29, 2015·Stem Cells and Development·Alexander HelmSylvia Ritter
Apr 1, 2008·Experimental Cell Research·Elisia D Tichy, Peter J Stambrook
Mar 25, 2016·Nature Communications·Sohee JunJae-Il Park
Feb 6, 2004·Journal of Biochemistry and Molecular Biology·Chunming Ding, Charles R Cantor
Feb 6, 2004·Journal of Biochemistry and Molecular Biology·Young Hoon SungHan-Woong Lee
May 23, 2008·Radiation Research·Kai RothkammMarkus Löbrich
Mar 14, 2013·Trends in Molecular Medicine·Eugene D Sverdlov, Konstantin Mineev
Jan 21, 2003·Genesis : the Journal of Genetics and Development·Hanna H J ChaoYun You
Oct 5, 2001·Nature Reviews. Genetics·Y Yu, A Bradley
Dec 5, 2002·Physiological Genomics·Louise van der WeydenAllan Bradley
Jul 15, 2020·Cells·Christine E HellwegAgapios Sachinidis
Aug 1, 1998·Genome Research·J Schimenti, M Bucan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.