PMID: 2122527Sep 1, 1990Paper

X-ray induction of methotrexate resistance due to dhfr gene amplification

Somatic Cell and Molecular Genetics
P J HahnW F Morgan

Abstract

The effect of ionizing radiation on methotrexate (MTX) resistance and gene amplification in cultured mammalian cells was investigated. X-irradiation of mouse EMT-6 cells induced cell killing and MTX resistance due to amplification of dihydrofolate reductase (dhfr) gene in a dose-dependent manner. The highest yields of mutant cells were obtained at approximately D37 (the dose at which 37% of the cells survive), where the frequency of MTX-resistant cells was four- to eightfold over that of the unirradiated population. The proportion of MTX-resistant cells among the survivors increased logarithmically with dose, up to a 1000-fold increase over unirradiated cells at 1000 cGy, the highest dose tested. The induced frequency of MTX resistance after X-irradiation was greater than the induced frequency of 8-azaguanine resistance, which indicates deletion of the hypoxanthine phosphoribosyltransferase gene. Inhibition of poly(ADP-ribose) polymerase by the addition of 3-aminobenzamide before irradiation increased both cell killing and MTX resistance. Metaphase spreads of chromosomes from EMT-6 cells that had been irradiated and subjected to stepwise increases in MTX concentration showed numerous double minutes. Pulsed-field gel electrophor...Continue Reading

References

Sep 1, 1976·Journal of the National Cancer Institute·J L Biedler, B A Spengler
Nov 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J H NunbergL A Chasin
Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·R J KaufmanR T Schimke
Apr 1, 1988·Molecular and Cellular Biology·S M CarrollG M Wahl
Feb 27, 1987·Biochemical and Biophysical Research Communications·J K Wiencke, W F Morgan
Jun 10, 1988·Nucleic Acids Research·A M van der BliekP Borst
Jan 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·A J GrosovskyB W Glickman
Nov 1, 1987·Somatic Cell and Molecular Genetics·P HahnR B Painter
Jan 1, 1985·Pharmacology & Therapeutics·J E CleaverW F Morgan
Oct 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S Lavi
Apr 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G M Church, W Gilbert
Mar 1, 1984·Cancer Treatment Reviews·R T SchimkeE Mosse
Jul 1, 1984·Cell·R T Schimke
Jan 1, 1984·Annual Review of Biochemistry·G R Stark, G M Wahl
Jan 1, 1984·International Review of Cytology·J L HamlinJ C Azizkhan
May 1, 1983·Cell·J M RobertsR Axel
Jan 1, 1980·Environmental Mutagenesis·W F Morgan, P E Crossen
Jan 1, 1980·Human Genetics·P E Crossen, W F Morgan

❮ Previous
Next ❯

Citations

Mar 1, 1995·Cancer Metastasis Reviews·W F Morgan, J P Murnane
Jan 20, 2005·European Journal of Nuclear Medicine and Molecular Imaging·Michel H BourguignonEdgardo D Carosella
Feb 1, 1993·Mutation Research·M RoyS B Bhattacharjee
May 1, 1992·Mutation Research·M E PerryG R Stark
Feb 1, 1992·Genetic Analysis, Techniques and Applications·P J HahnJ Hozier
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·K Grube, A Bürkle
Jan 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Bin YanChuan-Yuan Li
Mar 1, 1993·Mutation Research·A Di LeonardoA Maddalena
May 1, 1994·International Journal of Hyperthermia : the Official Journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group·B NevaldineP J Hahn
Feb 28, 2008·Radiation Research·Robert R KimmelJeffrey L Schwartz
Sep 2, 1998·Genomics·R Rizwana, P J Hahn
Dec 18, 2001·Environmental and Molecular Mutagenesis·M BerwickI Orlow
Jul 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P J Hahn
Oct 23, 1997·International Journal of Cancer. Journal International Du Cancer·R M HarvieR A Davey
Sep 16, 2020·Journal of Hematology & Oncology·Yuanliang YanZhijie Xu
Apr 1, 1996·Journal of Clinical Laser Medicine & Surgery·A P LoRussoR J Lanzafame
Dec 26, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Axel WeberHolger Christiansen
Feb 17, 2001·Biochemical and Biophysical Research Communications·H S KimC H Choi
Nov 1, 1993·Molecular and Cellular Biology·B A Marder, W F Morgan
Aug 1, 1993·Molecular and Cellular Biology·J L BelandP J Hahn
Jun 1, 1992·Molecular and Cellular Biology·M B Benjamin, J B Little

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.