X-ray structure and microtubule interaction of the motor domain of Neurospora crassa NcKin3, a kinesin with unusual processivity

Biochemistry
Alexander MarxEckhard Mandelkow

Abstract

Neurospora crassa kinesin NcKin3 belongs to a unique fungal-specific subgroup of small Kinesin-3-related motor proteins. One of its functions appears to be the transport of mitochondria along microtubules. Here, we present the X-ray structure of a C-terminally truncated monomeric construct of NcKin3 comprising the motor domain and the neck linker, and a 3-D image reconstruction of this motor domain bound to microtubules, by cryoelectron microscopy. The protein contains Mg.ADP bound to the active site, yet the structure resembles an ATP-bound state. By comparison with structures of the Kinesin-3 motor Kif1A in different nucleotide states (Kikkawa, M. et al. (2001) Nature (London, U.K.) 411, 439-445), the NcKin3 structure corresponds to the AMPPCP complex of Kif1A rather than the AMPPNP complex. NcKin3-specific differences in the coordination of the nucleotide and asymmetric interactions between adjacent molecules in the crystal are discussed in the context of the unusual kinetics of the dimeric wild-type motor and the monomeric construct used for crystal structure analysis. The NcKin3 motor decorates microtubules at a stoichiometry of one head per alphabeta-tubulin heterodimer, thereby forming an axial periodicity of 8 nm. In sp...Continue Reading

References

May 1, 1988·Quarterly Reviews of Biophysics·J DubochetP Schultz
Jan 1, 1996·Journal of Structural Biology·J P Schroeter, J P Bretaudiere
Feb 16, 1999·Cell·E NogalesK H Downing
Apr 15, 2000·Journal of Molecular Biology·A HoengerE Mandelkow
Nov 12, 2002·The EMBO Journal·Thomas G WendtAndreas Hoenger
Mar 28, 2003·The EMBO Journal·Georgios SkiniotisAndreas Hoenger
May 14, 2003·Fungal Genetics and Biology : FG & B·Conrad L SchochB Gillian Turgeon
Nov 26, 2003·The Journal of Cell Biology·Jawdat Al-BassamRonald A Milligan
Dec 6, 2003·Journal of Cell Science·Elise M Dagenbach, Sharyn A Endow
Dec 9, 2003·Journal of Molecular Biology·A KrebsA Hoenger
Feb 21, 2004·The EMBO Journal·Georgios SkiniotisAndreas Hoenger
Feb 28, 2004·The Journal of Biological Chemistry·Maia V VinogradovaRobert J Fletterick
Jul 17, 2004·The EMBO Journal·Hideo HiguchiSharyn A Endow
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Oct 14, 2004·The Journal of Cell Biology·Carolyn J LawrenceLinda Wordeman
May 12, 2005·The Journal of Peptide Research : Official Journal of the American Peptide Society·D J RashidD W Pierce
Dec 20, 2005·Current Opinion in Cell Biology·Nicholas J Carter, Robert A Cross
Oct 3, 2006·The Journal of Biological Chemistry·Sarah AdioGünther Woehlke
Dec 26, 2006·Current Opinion in Cell Biology·Megan T Valentine, Susan P Gilbert
May 2, 2007·The Journal of Cell Biology·Charles V Sindelar, Kenneth H Downing

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Citations

Oct 28, 2009·The Journal of Biological Chemistry·Elzbieta KocikAndrzej A Kasprzak
Feb 27, 2009·PloS One·Sarah AdioGünther Woehlke
Jun 17, 2009·Cell Motility and the Cytoskeleton·Alexander MarxEckhard Mandelkow
Jun 6, 2009·The FEBS Journal·Sarah Adio, Günther Woehlke
Feb 1, 2011·Journal of Molecular Biology·Nariman NaberEdward Pate
Oct 19, 2005·Advances in Protein Chemistry·John M Squire, David A D Parry
Nov 28, 2009·The British Journal of Sociology·David Tyfield, John Urry
May 31, 2020·Progress in Nuclear Magnetic Resonance Spectroscopy·Thomas Wiegand
Nov 20, 2021·Science Advances·April L SolonMichael A Cianfrocco

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