X-ray structure of the MMTV-A nucleosome core

Proceedings of the National Academy of Sciences of the United States of America
Timothy D FrouwsT Richmond

Abstract

The conformation of DNA bound in nucleosomes depends on the DNA sequence. Questions such as how nucleosomes are positioned and how they potentially bind sequence-dependent nuclear factors require near-atomic resolution structures of the nucleosome core containing different DNA sequences; despite this, only the DNA for two similar α-satellite sequences and a sequence (601) selected in vitro have been visualized bound in the nucleosome core. Here we report the 2.6-Å resolution X-ray structure of a nucleosome core particle containing the DNA sequence of nucleosome A of the 3'-LTR of the mouse mammary tumor virus (147 bp MMTV-A). To our knowledge, this is the first nucleosome core particle structure containing a promoter sequence and crystallized from Mg(2+) ions. It reveals sequence-dependent DNA conformations not seen previously, including kinking into the DNA major groove.

References

Feb 1, 1989·Journal of Biomolecular Structure & Dynamics·R Lavery, H Sklenar
Oct 25, 1982·Journal of Molecular Biology·C R Calladine
May 31, 1996·Journal of Molecular Biology·M A el Hassan, C R Calladine
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Jun 25, 2002·Journal of Molecular Biology·Curt A DaveyTimothy J Richmond
May 9, 2003·Nature·Timothy J Richmond, Curt A Davey
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Nov 30, 2004·Science·Benedetta DorigoTimothy J Richmond
Dec 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Philip Evans
Mar 24, 2007·Journal of Molecular Biology·Michelle S OngCurt A Davey
Nov 17, 2007·Nature Protocols·Axel T Brunger
May 6, 2008·Acta Crystallographica. Section D, Biological Crystallography·Thomas C TerwilligerLi-Wei Hung
Feb 11, 2009·Nature Reviews. Genetics·Cizhong Jiang, B Franklin Pugh
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Jun 11, 2009·Nucleic Acids Research·Alexandre V MorozovEric D Siggia
Jul 15, 2009·Trends in Genetics : TIG·Eran Segal, Jonathan Widom
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Wolfgang Kabsch
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Aug 31, 2010·Journal of Molecular Biology·Dileep VasudevanCurt A Davey
Mar 29, 2012·Nucleic Acids Research·Eugene Y D ChuaCurt A Davey
Jun 23, 2012·Nature·Kristin BrogaardJonathan Widom
Feb 20, 2013·Current Opinion in Structural Biology·Curt Alexander Davey
Mar 7, 2013·Nature Structural & Molecular Biology·Kevin Struhl, Eran Segal
May 8, 2013·Proceedings of the National Academy of Sciences of the United States of America·Feng WangDanesh Moazed
Dec 17, 2014·Chemical Reviews·Robert K McGinty, Song Tan
Jun 11, 2015·Nature·Daniel P MaskellPeter Cherepanov

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Citations

Aug 30, 2016·Cell Biology International·Maria A Kuznetsova, Eugene V Sheval
Nov 22, 2017·Journal of Biochemistry·Masako Koyama, Hitoshi Kurumizaka
Sep 28, 2016·Journal of Computational Chemistry·Steven C HowellJoseph E Curtis
Jul 29, 2020·Essays in Biochemistry·Ahmad Ali-Ahmad, Nikolina Sekulić
Jan 27, 2018·Acta Crystallographica. Section D, Structural Biology·Bohdan SchneiderJiří Černý
Mar 23, 2018·Open Biology·Yoshimasa TakizawaHitoshi Kurumizaka
May 7, 2020·Nucleic Acids Research·Aghil SomanLars Nordenskiöld
May 20, 2018·Physical Review. E·Aaron M MorganChristopher J Fischer
Feb 13, 2020·Communications Biology·Risa FujitaHitoshi Kurumizaka
Jan 30, 2021·EMBO Reports·Lara Zorro ShahidianRobert Schneider
Dec 31, 2020·International Journal of Molecular Sciences·Aaron MorganChristopher Fischer
Oct 17, 2020·Journal of Molecular Biology·Hitoshi KurumizakaYoshimasa Takizawa
Dec 14, 2016·Biophysical Journal·Stefjord TodolliWilma K Olson
Sep 7, 2019·Journal of Chemical Information and Modeling·Ran SunThomas C Bishop
Nov 28, 2018·The Journal of Physical Chemistry. B·Kaushik ChakrabortySharon M Loverde
Oct 1, 2021·Chemical Reviews·Eric LargyValérie Gabelica

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