PMID: 9659395Jul 11, 1998Paper

X-ray studies on cross-linked lysozyme crystals in acetonitrile-water mixture

Biochimica Et Biophysica Acta
Z WangY Tang

Abstract

Tetragonal crystals of hen egg white lysozyme were cross-linked and subjected to X-ray diffraction study in acetonitrile-water media with different acetonitrile concentrations. Crystals in neat acetonitrile did not scatter X-ray well. Structures of crystals in neat water, in 90% and 95% acetonitrile, and crystal back-soaked from acetonitrile to water, were determined to about 2 A resolution. For crystals in both 90% acetonitrile, and crystal back-soaked from acetonitrile to water, were determined to about 2 A resolution. For crystals in both 90% and 95% acetonitrile, only one protein-bond acetonitrile molecule is found in the active site cleft, and its location and binding-protein mode is similar to the C subunit of polysaccharide. The alteration in conformation and hydrogen-bond pattern involving water as solvent causes the reduction of the protein's flexibility in organic media. The back-soaked crystal regained its ordinary three-dimensional structure in water.

References

Oct 5, 1975·Journal of Molecular Biology·C R BeddellS J Oatley
Feb 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R AffleckJ S Dordick
Jan 15, 1992·European Journal of Biochemistry·M N Gupta
May 1, 1986·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H OkamotoT Nakamura
Apr 1, 1989·Trends in Biochemical Sciences·A M Klibanov
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S B Ha, G An
Nov 1, 1988·European Journal of Biochemistry·M ReslowB Mattiasson
Jan 5, 1987·Journal of Molecular Biology·C E Kundrot, F M Richards
Jan 25, 1974·Journal of Molecular Biology·R Diamond
Jan 25, 1974·Journal of Molecular Biology·M Levitt
Jun 14, 1994·Biochemistry·N H YennawarG K Farber
Jan 1, 1994·Trends in Biochemical Sciences·C M DobsonS E Radford
May 18, 1994·Biochimica Et Biophysica Acta·C R Wescott, A M Klibanov
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P A FitzpatrickA M Klibanov
Feb 1, 1993·Protein Engineering·T UedaT Imoto
Sep 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·F A QUIOCHO, F M RICHARDS

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Citations

Mar 5, 2003·Journal of Molecular Biology·N MorelletB P Roques
Feb 9, 2000·Pharmacology & Therapeutics·G K Farber
May 9, 2001·Biochimica Et Biophysica Acta·V V GorbatchukB N Solomonov
Feb 18, 1999·Current Opinion in Chemical Biology·D Häring, P Schreier
May 10, 2012·Quarterly Reviews of Biophysics·Katrina W Lexa, Heather A Carlson
Dec 17, 2010·Journal of the American Chemical Society·Katrina W Lexa, Heather A Carlson
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·X G GaoA Rodríguez-Romero
Sep 12, 2013·PloS One·Pemra OzbekTurkan Haliloglu
Mar 31, 2006·Biotechnology and Bioengineering·Noa Cohen-HadarAmihay Freeman
Jul 14, 2001·Biochimica Et Biophysica Acta·G ZhuY Tang
Jan 27, 1999·Biochimica Et Biophysica Acta·G ZhuY Tang
Jun 21, 2011·Journal of the American Chemical Society·John L KulpFrank Guarnieri
Jun 10, 2014·Journal of Chemical Theory and Computation·Daniel Alvarez-Garcia, Xavier Barril
Nov 19, 2011·Journal of the American Chemical Society·David H HallSandor Vajda
Dec 10, 2013·Journal of Chemical Theory and Computation·Guanglei CuiEric S Manas

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