X-rays Reveal the Internal Structure of Keratin Bundles in Whole Cells

ACS Nano
Clément Y J HémonnotSarah Köster

Abstract

In recent years, X-ray imaging of biological cells has emerged as a complementary alternative to fluorescence and electron microscopy. Different techniques were established and successfully applied to macromolecular assemblies and structures in cells. However, while the resolution is reaching the nanometer scale, the dose is increasing. It is essential to develop strategies to overcome or reduce radiation damage. Here we approach this intrinsic problem by combing two different X-ray techniques, namely ptychography and nanodiffraction, in one experiment and on the same sample. We acquire low dose ptychography overview images of whole cells at a resolution of 65 nm. We subsequently record high-resolution nanodiffraction data from regions of interest. By comparing images from the two modalities, we can exclude strong effects of radiation damage on the specimen. From the diffraction data we retrieve quantitative structural information from intracellular bundles of keratin intermediate filaments such as a filament radius of 5 nm, hexagonal geometric arrangement with an interfilament distance of 14 nm and bundle diameters on the order of 70 nm. Thus, we present an appealing combined approach to answer a broad range of questions in so...Continue Reading

References

Jan 1, 1985·Annals of the New York Academy of Sciences·R EichnerU Aebi
May 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A C StevenP M Steinert
Aug 1, 1982·Journal of Microscopy·W O Saxton, W Baumeister
Jan 1, 1994·International Journal of Radiation Biology·P A Riley
Jul 1, 1993·Fortschritte der Neurologie-Psychiatrie·J Bauer, M Berger
Jan 1, 1996·Connective Tissue Research·P FratzlK Klaushofer
Sep 30, 1996·Physical Review Letters·K A NugentZ Barnea
Mar 9, 2004·Molecular Biology of the Cell·Reinhard WindofferRudolf E Leube
Sep 18, 2004·The Journal of Investigative Dermatology·Lars Norlén, Ashraf Al-Amoudi
Aug 30, 2005·Journal of Structural Biology·Marin van Heel, Michael Schatz
Jul 21, 2006·The Journal of Physical Chemistry. B·S FörsterR Knott
May 8, 2007·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Bert MüllerPhilipp J Thurner
May 6, 2008·Microscopy Research and Technique·Guk Bae KimSang Joon Lee
Feb 10, 2009·Ultramicroscopy·Pierre ThibaultFranz Pfeiffer
Mar 31, 2009·Osteoarthritis and Cartilage·J LiC Muehleman
Apr 23, 2009·Journal of Molecular Biology·Cristiano Luis Pinto OliveiraJan Skov Pedersen
Jun 23, 2009·Ultramicroscopy·Andrew M Maiden, John M Rodenburg
Dec 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Klaus GiewekemeyerTim Salditt
Mar 1, 2009·Journal of Electron Spectroscopy and Related Phenomena·M R HowellsD Starodub
Jul 21, 2010·The Journal of Cell Biology·Lothar SchermellehHeinrich Leonhardt
Jul 28, 2010·Journal of the Royal Society, Interface·Georg SchulzBert Müller
Apr 26, 2011·NeuroImage·T H JensenF Pfeiffer
Nov 17, 2011·Journal of Structural Biology·Tanja LichtensternHarald Herrmann
Dec 5, 2012·Lab on a Chip·Britta Weinhausen, Sarah Köster
Jan 22, 2013·Biophysical Journal·Steve P MeisburgerRobert E Thorne
Oct 30, 2013·Proceedings of the National Academy of Sciences of the United States of America·Lena RammsBernd Hoffmann
Aug 26, 2014·Nature Communications·Andrew V MartinHenry N Chapman
Nov 22, 2014·ACS Nano·Valeria PiazzaSarah Köster
Dec 4, 2014·Biophysical Journal·Marius PriebeTim Salditt
Dec 4, 2014·Biophysical Journal·Jens-Friedrich NoltingSarah Köster
Feb 13, 2015·Proceedings of the National Academy of Sciences of the United States of America·Junjing DengChris J Jacobsen
Feb 14, 2015·Physical Review Letters·M BartelsT Salditt
Feb 4, 2016·Biophysical Journal·Marten BernhardtFlorian Rehfeldt

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Citations

Oct 13, 2017·Journal of the Royal Society, Interface·Tanya ProzorovRafal E Dunin-Borkowski
Jul 6, 2019·Journal of Synchrotron Radiation·Marten BernhardtTim Salditt
Sep 7, 2019·Journal of Synchrotron Radiation·Julio Cesar da SilvaPeter Cloetens
Oct 28, 2019·Essays in Biochemistry·Sungjun Yoon, Rudolf E Leube
Feb 7, 2018·Optics Express·Max RoseIvan A Vartanyants
Jul 28, 2016·Polymers·Jörg SchnaußJosef A Käs
Oct 25, 2017·Scientific Reports·Natalja StrelnikovaThomas Pfohl
Feb 11, 2021·Journal of Synchrotron Radiation·Chiara CassiniSarah Köster
Apr 6, 2019·Acta Biomaterialia·Wei HuangJoanna McKittrick
Dec 28, 2016·ACS Nano·Clément Y J HémonnotSarah Köster
Aug 9, 2017·ACS Nano·Clément Y J Hémonnot, Sarah Köster
Dec 28, 2017·ACS Nano·Gaetano CampiAntonio Bianconi

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