XANES investigation of the Co oxidation state in solution and in cancer cells treated with Co(III) complexes

Journal of Inorganic Biochemistry
Paul D BonnitchaTrevor W Hambley

Abstract

XANES spectroscopy has been used to investigate whether it is possible to determine the oxidation state and coordination environment of Co complexes following treatment of cancer cells with Co(III) or Co(II) complexes. Our results show that the variation of the XANES with coordination geometry make it impossible to do this in a completely reliable way which is in contrast to the situation for platinum and chromium. It was established that the XANES spectrum obtained from cells treated with [Co(diNOsar)]Br(3) remained unchanged with respect to its XANES spectrum obtained in solution, demonstrating that the [Co(diNOsar)]Br(3) complex remained intact after 24h in cellular media (diNOsar=1,8-dinitro-3,6,10,13,16,19-hexaazabicyclo[6.6.6]eicosane). In contrast, the XANES spectra obtained from cells treated with Na[Co(acac)(3)] and [Co(acac)(3)] differed from the XANES spectra of the respective complexes obtained in solution, indicating a change in co-ordination environment for both complexes upon uptake in cells. The similarity of these spectra suggests that appearance of this XANES can be used as an indication of loss of the carrier ligands, a useful indicator in the study of hypoxia selective complexes. The results obtained for Na[...Continue Reading

References

Jan 1, 1986·Annual Review of Biochemistry·D H Hamer
May 15, 1994·International Journal of Radiation Oncology, Biology, Physics·W A DennyS M Pullen
May 15, 1994·International Journal of Radiation Oncology, Biology, Physics·W R WilsonD C Ware
May 30, 2001·Journal of Inorganic Biochemistry·P J BlowerY Zheng
Oct 16, 2001·European Journal of Medicinal Chemistry·W A Denny
Jun 19, 2003·Journal of the American Chemical Society·Matthew D HallTrevor W Hambley
Jul 29, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Matthew D HallTrevor W Hambley
Feb 18, 2004·Chemical Research in Toxicology·Tsui-Chun TsouLouis W Chang

❮ Previous
Next ❯

Citations

Sep 24, 2009·Chemical Reviews·Reagan McRaeChristoph J Fahrni
Feb 1, 2011·Antioxidants & Redox Signaling·Ute JungwirthPetra Heffeter
Jan 9, 2007·Journal of Inorganic Biochemistry·Ramasamy IndumathyBalachandran Unni Nair
Nov 27, 2018·Nucleosides, Nucleotides & Nucleic Acids·Chittanahalli N SudhamaniManju Giridhar
Sep 11, 2007·Dalton Transactions : an International Journal of Inorganic Chemistry·Matthew D HallTrevor W Hambley
Nov 10, 2007·Dalton Transactions : an International Journal of Inorganic Chemistry·Trevor W Hambley
May 31, 2019·Chemical Communications : Chem Comm·Carlos Sanchez-CanoPeter J Sadler
Jun 2, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Tyler M OzvatJoseph M Zadrozny

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.