Xanthine dehydrogenase AtXDH1 from Arabidopsis thaliana is a potent producer of superoxide anions via its NADH oxidase activity.

Plant Molecular Biology
Maryam ZarepourFlorian Bittner

Abstract

Xanthine dehydrogenase AtXDH1 from Arabidopsis thaliana is a key enzyme in purine degradation where it oxidizes hypoxanthine to xanthine and xanthine to uric acid. Electrons released from these substrates are either transferred to NAD(+) or to molecular oxygen, thereby yielding NADH or superoxide, respectively. By an alternative activity, AtXDH1 is capable of oxidizing NADH with concomitant formation of NAD(+) and superoxide. Here we demonstrate that in comparison to the specific activity with xanthine as substrate, the specific activity of recombinant AtXDH1 with NADH as substrate is about 15-times higher accompanied by a doubling in superoxide production. The observation that NAD(+) inhibits NADH oxidase activity of AtXDH1 while NADH suppresses NAD(+)-dependent xanthine oxidation indicates that both NAD(+) and NADH compete for the same binding-site and that both sub-activities are not expressed at the same time. Rather, each sub-activity is determined by specific conditions such as the availability of substrates and co-substrates, which allows regulation of superoxide production by AtXDH1. Since AtXDH1 exhibits the most pronounced NADH oxidase activity among all xanthine dehydrogenase proteins studied thus far, our results im...Continue Reading

References

Sep 15, 1992·Biochimica Et Biophysica Acta·R Pérez-VicenteM Pineda
Sep 6, 1967·Biochimica Et Biophysica Acta·E J Landon, M Myles
Sep 30, 1969·Biochimica Et Biophysica Acta·E Della CorteF Stirpe
Feb 10, 1995·The Journal of Biological Chemistry·A SatoT Nishino
May 1, 1997·European Journal of Biochemistry·S A SandersR Harrison
Sep 27, 2000·Proceedings of the National Academy of Sciences of the United States of America·C EnrothE F Pai
Feb 24, 2001·Molecular Plant-microbe Interactions : MPMI·R SantosA Puppo
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·D B DattaE H Newcomb
Aug 23, 2002·The American Journal of Gastroenterology·Fiorenzo StirpeGian Luca Grazi
Sep 5, 2002·Free Radical Biology & Medicine·Roger Harrison
Feb 1, 1997·Plant Physiology·G. M. Pastori, L. A. Del Rio
Apr 1, 1996·Plant Physiology·P. R. EscuredoM. Becana
Jun 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yoshimitsu KuwabaraTakeshi Nishino
Jun 26, 1954·Nature·P G AVISK V SHOOTER
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Chris Lamb, Richard A. Dixon
Sep 30, 2004·Journal of Dairy Science·Mustapha BenboubetraRoger Harrison
Dec 16, 2004·Molecular Plant-microbe Interactions : MPMI·Maria C RubioManuel Becana
Oct 1, 1939·The Biochemical Journal·H S CorranD E Green
Feb 13, 2008·The Plant Journal : for Cell and Molecular Biology·Galina BrychkovaMoshe Sagi
Jun 10, 2008·Journal of Plant Physiology·Francisco J CorpasLuis A Del Río

❮ Previous
Next ❯

Citations

Jun 11, 2011·Plant Cell Reports·Ralf R Mendel
Oct 16, 2012·Plant Molecular Biology·Maryam ZarepourFlorian Bittner
Apr 18, 2013·Asian Pacific Journal of Tropical Biomedicine·Badakhshan Mahdi-PourSreenivasan Sasidharan
Nov 13, 2013·Journal of Experimental Botany·Panagiotis N Moschou, Kalliopi A Roubelakis-Angelakis
Jan 1, 2015·Plants·Ivan JajicKazimierz Strzalka
Oct 15, 2011·Phytochemistry·Oscar NavarreteSergei Storozhenko
Apr 13, 2011·Trends in Plant Science·Andrea K Werner, Claus-Peter Witte
Mar 12, 2016·Journal of Plant Physiology·Bikash BaralMaria Luisa Izaguirre-Mayoral
Mar 1, 2012·Biochimica Et Biophysica Acta·Ralf R Mendel, Tobias Kruse
Jan 1, 2015·Applied Microbiology and Biotechnology·Jung Chul ParkHeung-Shick Lee
Apr 4, 2014·Chemical Reviews·Luisa B Maia, José J G Moura
Aug 19, 2016·Bioengineered·Cheng-Hua WangXin-Hui Xing
Apr 12, 2011·Journal of Agricultural and Food Chemistry·Kevin A FordJohn E Casida
Jan 5, 2019·Journal of Integrative Plant Biology·Francisco J CorpasJosé M Palma
May 31, 2011·Metallomics : Integrated Biometal Science·Angel LlamasAurora Galván
Jun 27, 2013·Metallomics : Integrated Biometal Science·Manuel Tejada-JiménezÁngel Llamas
Mar 6, 2018·Frontiers in Cell and Developmental Biology·Dacheng Liang
Jul 19, 2017·Journal of Enzyme Inhibition and Medicinal Chemistry·Maria Elisa Melo Branco de AraújoPatricia de Oliveira Carvalho
May 28, 2019·Frontiers in Plant Science·Wei WangJi-Hong Liu
Jul 29, 2020·Frontiers in Plant Science·Francisco J CorpasJosé M Palma
Mar 7, 2021·Proceedings of the National Academy of Sciences of the United States of America·Ángela RománMichael J Haydon
Jun 8, 2017·Plant Physiology and Biochemistry : PPB·Junhuan XuFiona L Goggin
May 20, 2021·Journal of Experimental Botany·Su Yin PhuaFrank Van Breusegem
Jan 16, 2022·Environmental Science and Pollution Research International·Zhen-Xing WuJian Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.