PMID: 2495387Apr 1, 1989Paper

Xanthine oxidase-derived oxygen radicals induce pulmonary edema via direct endothelial cell injury

The Journal of Surgical Research
M A GrossoA H Harken

Abstract

Pulmonary hypoperfusion/ischemia-reperfusion (I/R) may initiate ARDS (nonhydrostatic pulmonary edema). Endothelial damage via xanthine oxidase (XO)-derived oxygen radicals (O2*) may mediate I/R injury. We previously documented Factor VIII antigen (F8) as a marker for endothelial injury. The purpose of this study was to (1) document I/R-induced nonhydrostatic pulmonary edema, (2) identify whether XO or O2* mediates nonhydrostatic edema, and (3) identify the site of injury (? endothelium). Rat lungs were isolated, ventilated, and perfused (100 min, control, or 40 min at 37 degrees C, I (static vent.), + 60 min, R). Effluent was analyzed for F8 release (ELISA: data relative to control). Tungsten-fed rats had negligible lung XO vs rats fed standard diet (3.6 vs 34.5 mU/g, (P less than 0.05). Catalase (CAT) 50 micrograms/ml) was added to perfusate prior to R. Sectioned lungs were fluorescein anti-F8 photographed (IF) and qualitatively assessed. (Table: see text). We conclude that (1) pulmonary hypoperfusion (I/R) leads to nonhydrostatic pulmonary edema, and (2) the edema results in part from XO-generated O2* directed at the capillary endothelium.

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Citations

Feb 19, 1991·Biochimica Et Biophysica Acta·P C PanusB A Freeman
Oct 13, 1998·The Annals of Thoracic Surgery·A O HalldorssonD Sidle
Aug 26, 1998·Journal of the American College of Cardiology·B S CainA H Harken
Apr 1, 1995·Journal of Sports Sciences·H B NielsenN H Secher
Nov 1, 2003·Antioxidants & Redox Signaling·Rohit BudhirajaPaul M Hassoun
Jul 1, 1995·Pharmacology & Toxicology·M SakaiT Tanaka
Nov 14, 1997·American Journal of Respiratory and Critical Care Medicine·E N AtochinaA B Fisher
Mar 1, 1991·The American Review of Respiratory Disease·A G LjungmanM L Morganroth
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May 11, 1991·Toxicology and Applied Pharmacology·H R AmouzadehA Mauromoustakos
Oct 6, 1997·Surgery·B S CainA H Harken
Sep 11, 1991·Journal of Pediatric Surgery·R E KellyT R Hall
Oct 1, 1996·The Journal of Thoracic and Cardiovascular Surgery·M A HidalgoC J Green
Mar 15, 2003·Neurological Research·Thomas E CeremugaJoseph T McCabe
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May 1, 1992·Archives of Biochemistry and Biophysics·P C PanusB A Freeman

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