Xanthohumol exhibits anti-myeloma activity in vitro through inhibition of cell proliferation, induction of apoptosis via the ERK and JNK-dependent mechanism, and suppression of sIL-6R and VEGF production

Biochimica Et Biophysica Acta. General Subjects
Adrianna Sławińska-BrychMariusz Gagoś

Abstract

Xanthohumol (XN, a hop-derived prenylflavonoid) was found to exert anticancer effects on various cancer types. However, the mechanisms by which XN affects the survival of multiple myeloma cells (MM) are little known. Therefore, our study was undertaken to address this issue. Anti-proliferative activity of XN towards two phenotypically distinct MM cell lines U266 and RPMI8226 was evaluated with the MTT and BrdU assays. Cytotoxicity was determined with the LDH method, whereas apoptosis was assessed by flow cytometry and fluorescence staining. The expression of cell cycle- and apoptosis-related proteins and the activation status of signaling pathways were estimated by immunoblotting and ELISA assays. XN reduced the viability of RPMI8226 cells more potently than in U266 cells. It blocked cell cycle progression through downregulation of cyclin D1 and increased p21 expression. The marked apoptosis induction in the XN-treated RPMI8226 cells was related to initiation of mitochondrial and extrinsic pathways, as indicated by the altered p53, Bax, and Bcl-2 protein expression, cleavage of procaspase 8 and 9, and elevated caspase-3 activity. The apoptotic process was probably mediated via ROS overproduction and MAPK (ERK and JNK) activatio...Continue Reading

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Citations

Jun 9, 2020·Frontiers in Cell and Developmental Biology·Shuying YinMee-Hyun Lee
Jan 16, 2021·Pharmaceutical Patent Analyst·Diwakar AggarwalHardeep S Tuli
Mar 13, 2021·Pharmacological Reports : PR·Kamal S AbdelrahmanMohamed Abdel-Aziz
Jun 3, 2021·Cancers·Radka MichalkovaJan Mojzis
May 18, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Oroitz Sánchez-AguinagaldeJose-Ramon Sarasua
Jun 26, 2021·IUBMB Life·Sosmitha GirisaAjaikumar B Kunnumakkara
Aug 25, 2021·Phytotherapy Research : PTR·Tingting XiaChanggang Sun

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