Xanthohumol Inhibits the Growth of Keratin 18-Overexpressed Esophageal Squamous Cell Carcinoma in vitro and in vivo

Frontiers in Cell and Developmental Biology
Shuying YinMee-Hyun Lee

Abstract

Esophageal squamous cell carcinoma (ESCC) is a leading cause of cancer-related death worldwide. Xanthohumol is a prenylated flavonoid isolated from hops. Although xanthohumol has been reported to exert anti-obesity, hypoglycemic, anti-hyperlipidemia and anti-cancer activities, the mechanisms underlying its chemotherapeutic activity are yet to be elucidated. In the present study, we found that xanthohumol inhibited ESCC cell proliferation in vitro and in vivo by targeting keratin (KRT)-18. Xanthohumol suppressed the proliferation, foci formation, and anchorage-independent colony growth of KYSE30 cells. Using xanthohumol-sepharose conjugated bead pull-down and mass/mass analysis, we found that KRT18 is a novel target of xanthohumol in KYSE30 cells. KRT18 protein was highly expressed in patient ESCC tissues compared to adjunct tissues. Anti-proliferative activity of xanthohumol was abrogated or enhanced according to the knockdown or overexpression of KRT18 protein, respectively. Xanthohumol also induced apoptosis and cell cycle arrest at G1 phase which was associated with the modulation of expression of related makers including cyclin D1, cyclin D3, and cleaved-PARP, Bcl-2, cytochrome c and Bax. While xanthohumol attenuated KRT18 ...Continue Reading

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Citations

Dec 18, 2020·Frontiers in Cell and Developmental Biology·Tingting LiuKangdong Liu
May 1, 2021·International Journal of Molecular Sciences·Vancha HarishAnupam Bishayee
Aug 17, 2021·Molecular Medicine Reports·Elsa N Garza-TreviñoSalvador L Said-Fernández

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Methods Mentioned

BETA
xenograft
Pull-Down
transfection
transfect
electrophoresis
xenografts

Software Mentioned

Image
Pro Plus

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