PMID: 9537354Apr 16, 1998Paper

Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence

Journal of Bacteriology
F KatzenLuis Ielpi

Abstract

Xanthan is an industrially important exopolysaccharide produced by the phytopathogenic, gram-negative bacterium Xanthomonas campestris pv. campestris. It is composed of polymerized pentasaccharide repeating units which are assembled by the sequential addition of glucose-1-phosphate, glucose, mannose, glucuronic acid, and mannose on a polyprenol phosphate carrier (L. Ielpi, R. O. Couso, and M. A. Dankert, J. Bacteriol. 175:2490-2500, 1993). A cluster of 12 genes in a region designated xpsI or gum has been suggested to encode proteins involved in the synthesis and polymerization of the lipid intermediate. However, no experimental evidence supporting this suggestion has been published. In this work, from the biochemical analysis of a defined set of X. campestris gum mutants, we report experimental data for assigning functions to the products of the gum genes. We also show that the first step in the assembly of the lipid-linked intermediate is severely affected by the combination of certain gum and non-gum mutations. In addition, we provide evidence that the C-terminal domain of the gumD gene product is sufficient for its glucosyl-1-phosphate transferase activity. Finally, we found that alterations in the later stages of xanthan bi...Continue Reading

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Citations

Sep 26, 2000·The Journal of Biological Chemistry·P L AbdianR A Geremia
Jul 29, 2016·Molecular Plant-microbe Interactions : MPMI·María Isabel BiancoAdrián A Vojnov
Sep 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·J Maxwell DowJi-Liang Tang
Jul 31, 2004·Annual Review of Phytopathology·Leandro M MoreiraAna C R da Silva
Oct 24, 2006·Nature Biotechnology·Andrea KrauseAlexander Goesmann
May 18, 2013·Molecular Plant-microbe Interactions : MPMI·N KillinyR P P Almeida
Dec 1, 2010·Current Protocols in Chemical Biology·Jennifer Campbell
Oct 3, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Melisa JacobsLuis Ielpi
Jun 16, 2015·Frontiers in Microbiology·Jochen SchmidBernd Rehm
Sep 30, 2016·Applied Microbiology and Biotechnology·Linxiang XuJianfa Zhang
Dec 10, 2017·EMBO Reports·Sreegowrinadh JavvadiSubhadeep Chatterjee
Nov 9, 2006·Biotechnology Letters·Ki-Hoon Yoon, Jae-Yong Cho
Jul 4, 2008·The Journal of Biological Chemistry·Máximo BarrerasLuis Ielpi
Nov 21, 2009·FEMS Microbiology Reviews·Daniela Büttner, Ulla Bonas
Sep 1, 2015·Molecular Plant Pathology·Minh-Phuong NguyenSang-Won Lee
Aug 2, 2014·Applied Microbiology and Biotechnology·Jochen SchmidVolker Sieber

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