PMID: 9555077Jun 20, 1998Paper

Xanthophyll biosynthesis: molecular and functional characterization of carotenoid hydroxylases from pepper fruits (Capsicum annuum L.)

Biochimica Et Biophysica Acta
F BouvierB Camara

Abstract

To dissect the mechanism by which carotenoid hydroxylases catalyze xanthophyll formation, we have cloned two pepper cDNAs encoding beta-cryptoxanthin and zeaxanthin biosynthetic enzymes. Using an in vitro system, we find that both enzymes are ferredoxin dependent and that their activity is strongly inhibited by iron chelators such as o-phenanthroline or 8-hydroxyquinoline. This suggests the transfer of a reducing equivalent from NADPH to the hydroxylase via ferredoxin and the involvement of an iron activated oxygen insertion process. Based on sequence analysis, the putative histidine clusters involved in the iron coordination were identified and their roles evaluated. Following site-directed mutagenesis of the identified histidine residues hydroxylase activity was totally inactivated. Collectively, our data indicate that carotenoid hydroxylases belong to a new class of diiron proteins structurally related to membrane fatty acid desaturases. Mechanistically, both types of enzymes exploit iron activated oxygen to break the C-H bond with concomitant formation of double bond or oxygen insertion. We propose that the same mechanism operates for beta-carotene ketolase and probably for other carotenoid oxygenases as well.

References

May 29, 1992·Biochemical and Biophysical Research Communications·P D Fraser, G Sandmann
Jun 28, 1991·Science·F H Arnold, B L Haymore
Feb 26, 1990·FEBS Letters·Y Gavel, G von Heijne
Mar 25, 1989·Nucleic Acids Research·T C VerwoerdA Hoekema
Apr 14, 1972·Biochemical and Biophysical Research Communications·R W Topham, J L Gaylor
Jan 11, 1984·Nucleic Acids Research·J DevereuxO Smithies
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·F J van de LooC Somerville
Nov 15, 1994·Molecular & General Genetics : MGG·B HundleJ E Hearst
Oct 1, 1993·Plant Physiology·N S YadavK L Stecca
Dec 1, 1995·Current Opinion in Structural Biology·P Nordlund, H Eklund
Oct 4, 1996·The Journal of Biological Chemistry·Z SunF X Cunningham
Mar 1, 1962·Archives of Biochemistry and Biophysics·H Y YAMAMOTOT O NAKAYAMA
Jul 2, 1962·Biochimica Et Biophysica Acta·J B MARSH, A T JAMES
Dec 31, 2008·The Journal of Biological Chemistry·Daniel LambDavid W Brighty

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Citations

Jun 14, 2000·Current Opinion in Biotechnology·C Schmidt-Dannert
Aug 17, 1999·Current Opinion in Structural Biology·C M SummaW F DeGrado
Apr 20, 2001·Current Opinion in Plant Biology·J Hirschberg
Jan 8, 2004·Proceedings of the National Academy of Sciences of the United States of America·Li TianDean DellaPenna
Oct 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·T A ThorupM Jahn
Nov 26, 2009·Journal of Experimental Botany·Chihiro YamamizoAkemi Ohmiya
May 24, 2013·Plant Physiology·Yun-Kyoung KimChoong-Ill Cheon
May 15, 2012·The Arabidopsis Book·M Águila Ruiz-Sola, Manuel Rodríguez-Concepción
Sep 12, 2014·Science China. Life Sciences·KaiJing ZhengZhangLi Hu
Aug 21, 2014·The Plant Journal : for Cell and Molecular Biology·Anna BerimDavid R Gang
Nov 22, 2005·Biochemical and Biophysical Research Communications·Kyoko MomoiVlada B Urlacher
Dec 1, 2004·Annual Review of Genetics·Arthur R GrossmanChung Soon Im
Aug 8, 2006·FEBS Letters·Alessia FioreGiovanni Giuliano
May 4, 2006·Annual Review of Plant Biology·Dean DellaPenna, Barry J Pogson
May 24, 2012·Journal of Natural Products·Mark A ScaifeRoberto E Armenta
Apr 4, 2012·Plant & Cell Physiology·Ming-Zhu LvHong-Xuan Lin
Jan 17, 2009·Plant & Cell Physiology·Joonyul KimDean Dellapenna
Jan 1, 2017·Nitric Oxide : Biology and Chemistry·Marta Rodríguez-RuizFrancisco J Corpas
Sep 1, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chinreddy Subramanyam ReddyAnd Yeon Bok Kim
May 23, 2002·FEBS Letters·Sridhar DharmapuriGiovanni Giuliano
Oct 12, 2005·Journal of Experimental Botany·Shanna MooreJames Giovannoni
Sep 11, 2010·Critical Reviews in Food Science and Nutrition·K K Namitha, P S Negi
Jul 13, 2019·Journal of Industrial Microbiology & Biotechnology·Yuanqing WuXueming Zhao
Oct 29, 2008·Genome Génome / Conseil National De Recherches Canada·Rong LiAbdelali Hannoufa
Feb 16, 2011·Natural Product Reports·Michael H Walter, Dieter Strack
Mar 28, 2003·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Karen BöhmeReimund Goss
Apr 28, 2020·Progress in Retinal and Eye Research·Johannes von LintigDarwin Babino
May 22, 2001·Archives of Biochemistry and Biophysics·G Sandmann
Nov 18, 2005·Progress in Lipid Research·Florence BouvierBilal Camara

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