XAS analysis of a nanostructured iron polysaccharide produced anaerobically by a strain of Klebsiella oxytoca

Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine
Iztok ArčonFranco Baldi

Abstract

A strain of Klebsiella oxytoca, isolated from acid pyrite-mine drainage, characteristically produces a ferric hydrogel, consisting of branched heptasaccharide repeating units exopolysaccharide (EPS), with metal content of 36 wt%. The high content of iron in the EPS matrix cannot be explained by a simple ferric ion bond to the sugar skeleton. The bio-generated Fe-EPS is investigated by X-ray absorption spectroscopy. Fe K-edge XANES analysis shows that iron is mostly in trivalent form, with a non-negligible amount of Fe(2+) in the structure. The Fe EXAFS results indicate that iron in the sample is in a mineralized form, prevalently in the form of nano-sized particles of iron oxides/hydroxides, most probably a mixture of different nano-crystalline forms. TEM shows that these nanoparticles are located in the interior of the EPS matrix, as in ferritin. The strain produces Fe-EPS to modulate Fe-ions uptake from the cytoplasm to avoid iron toxicity under anaerobic conditions. This microbial material is potentially applicable as iron regulator.

References

Jun 1, 1995·Journal of Inorganic Biochemistry·E M CoeR D Bereman
Dec 7, 1992·Physical Review Letters·J J RehrS I Zabinsky
Jul 14, 2001·FEMS Microbiology Ecology·F BaldiA Scozzafava
Jun 22, 2005·Journal of Synchrotron Radiation·B Ravel, M Newville
Jan 27, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Richard K Watt

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Citations

May 22, 2013·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Thanchanok KangwankraiphaisanGeoffrey Michael Gadd
Mar 29, 2020·Mycorrhiza·Pamela LeonardiLaura Chiarantini
Dec 1, 2017·Applied Microbiology and Biotechnology·Giada Giusi PicceriLaura Chiarantini
Dec 21, 2020·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Han YangDan Zheng
Oct 26, 2021·Journal of the American Chemical Society·Davide MengaTim-Patrick Fellinger

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