XBP1-Mediated BiP/GRP78 Upregulation Copes with Oxidative Stress in Mosquito Cells during Dengue 2 Virus Infection

BioMed Research International
Tien-Huang ChenWei-June Chen

Abstract

Dengue viruses (DENVs) cause dengue fever which is an important mosquito-borne disease in tropical areas. Generally, DENV does not cause cellular damage in mosquito cells. However, alterations in cytosolic calcium ions ([Ca2+]cyt) and the mitochondrial membrane potential (MMP), as well as accumulated reactive oxygen species (ROS), including superoxide anions (O2∙-) and hydrogen peroxide (H2O2), can be detected in C6/36 cells with DENV2 infection. Evident upregulation of BiP/GRP78 also appeared at 24 h postinfection in DENV2-infected C6/36 cells. As expression of BiP/GRP78 mRNA was reduced when the transcription factor X-box-binding protein-1 (XBP1) was knocked down in C6/36 cells, it demonstrated that BiP/GRP78 is the target gene regulated by the XBP1 signal pathway. We further demonstrated that the expression and splicing activity of XBP1 were upregulated in parallel with DENV2 infection in C6/36 cells. In C6/36 cells with BiP/GRP78 overexpression, oxidative stress indicators including [Ca2+]cyt, MMP, O2∙-, and H2O2 were all pushed back to normal. Taken together, DENV2 activates XBP1 at earlier stage of infection, followed by upregulating BiP/GRP78 in mosquito cells. This regulatory pathway contributes a cascade in relation to...Continue Reading

References

Dec 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J P Hendrick, F U Hartl
Dec 22, 1999·Seminars in Cell & Developmental Biology·M J Gething
Jun 20, 2001·Nature Reviews. Molecular Cell Biology·M J BerridgeM D Bootman
Jan 10, 2002·Cell·Y Ma, L M Hendershot
Sep 18, 2002·Archives of Medical Research·Duane J Gubler
Mar 4, 2003·Nature Reviews. Molecular Cell Biology·Lars Ellgaard, Ari Helenius
Apr 15, 2003·Journal of Cell Science·Chuan Yin Liu, Randal J Kaufman
Oct 16, 2003·Molecular and Cellular Biology·Ann-Hwee LeeLaurie H Glimcher
Dec 31, 2003·Advances in Virus Research·Brett D Lindenbach, Charles M Rice
Jan 30, 2004·Journal of Medical Virology·Thananya ThongtanDuncan R Smith
Apr 9, 2004·The Journal of Biological Chemistry·Kezhong Zhang, Randal J Kaufman
Apr 5, 2005·Methods : a Companion to Methods in Enzymology·Amy S Lee
Apr 4, 2006·The Journal of Eukaryotic Microbiology·Chin-Gi HuangWei-June Chen
Jun 15, 2006·The Journal of Toxicological Sciences·Morihiko HirotaSetsuya Aiba
Sep 1, 2007·Antioxidants & Redox Signaling·Ronald C Wek, Douglas R Cavener
Dec 9, 2008·The Biochemical Journal·Michael P Murphy
Dec 25, 2008·Biochemical and Biophysical Research Communications·Thawornchai LimjindapornPa-thai Yenchitsomanus
Apr 28, 2009·Nature·October M SessionsMariano A Garcia-Blanco
Jul 20, 2011·Parasites & Vectors·Sirilaksana PatramoolDorothée Missé
Jan 17, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mehdi HammadiFabien Van Coppenolle
Mar 23, 2013·Viruses·Charlène BraultBirke Bartosch
May 11, 2013·Biochimica Et Biophysica Acta·William M Nauseef
Jun 17, 2014·Journal of Proteomics·Ma Isabel SalazarVictoria Pando-Robles
Jul 6, 2014·Physiological Reviews·Dmitry B ZorovSteven J Sollott
Jul 2, 2015·PLoS Neglected Tropical Diseases·Chao-Fu YangWei-June Chen

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Citations

Mar 23, 2020·Journal of Cell Communication and Signaling·Shekufe Rezghi BarezMahmoud Aghaei
Sep 13, 2019·Cell Host & Microbe·Louis Lambrechts, Maria-Carla Saleh
Jun 28, 2020·Cellular Signalling·Khadeja-Tul KubraNektarios Barabutis

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Datasets Mentioned

BETA
AAEL005558-RA
KU672624
KU672623
DQ440225

Methods Mentioned

BETA
Assay
flow cytometry
fluorescence-activated cell sorter
transfection
PCR
electrophoresis
Immunoprecipitation
X-ray
Infection
confocal microscopy

Software Mentioned

7500

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