Xeno-Free Cultivation of Mesenchymal Stem Cells From the Corneal Stroma

Investigative Ophthalmology & Visual Science
Steffi MatthyssenNadia Zakaria

Abstract

The human cornea has recently been described as a source of corneal stroma-derived mesenchymal stem cells (hMSCs). In vitro expansion of these cells involves basal medium supplemented with fetal bovine serum (FBS). As animal-derived serum can confer a risk of disease transmission and can be subject to considerable lot-to-lot variability, it does not comply with newer Good Manufacturing Practice (GMP)-required animal component-free culture protocols for clinical translation. This study investigated animal-free alternatives to FBS for cultivation of human corneal stromal MSCs. Proliferative capacity was studied for cultures supplemented with different concentrations (2.5%, 5%, and 10%) of FBS, human AB serum, human platelet lysate (HPL), and XerumFree. Unsupplemented basal medium was used as a control. The expression of specific hMSC markers (CD73+, CD90+, CD105+, CD19-, CD34-, CD79α-, CD11b-, CD14-, CD45-, and HLA-DR-) and trilineage differentiation (adipogenesis, osteogenesis, and chondrogenesis) were compared for the two outperforming supplements: 10% FBS and HPL. HPL is the only consistent non-xeno supplement where hMSC cultures show significantly higher proliferation than the 10% FBS-supplemented cultures. Both FBS- and HPL-...Continue Reading

Citations

Jul 17, 2019·Investigative Ophthalmology & Visual Science·Aurelie Dos SantosSophie X Deng
Jun 3, 2021·Journal of Personalized Medicine·Alisa DomninaNikolay Nikolsky
Sep 7, 2021·Journal of Cellular and Molecular Medicine·Nina SeidelmannMatthias Fuest

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