Xeno-implantation of pig chondrocytes into rabbit to treat localized articular cartilage defects: an animal model

Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society
Manuel RamallalFrancisco J Blanco

Abstract

Articular cartilage has only a limited ability to regenerate. The transplantation of autologous chondrocytes is currently used to treat focal defects in human articular cartilage, although use of organs, tissues, or cells from different species is being investigated as an alternative treatment. The object of this study was to use xeno-transplantation of cultured pig chondrocytes for the repair of rabbit chondral defects, and to analyze the significance of tissue rejection in this animal model. Partial chondral defects, including removal of cartilage tissue and a part of the subchondral bone, were created in the lateral femoral condyles of 30 adult New Zealand White rabbits. A periosteal flap was sutured to the native cartilage with the cambium layer facing the defect. As a control, culture medium was injected into the defect void of one group of rabbits while in a treatment group, chondrocytes, isolated from normal femoral pig cartilage, were injected into the defect void. All rabbits were killed by 24 weeks. Macroscopic changes of the cartilage were analyzed using Mankin's score. The distal femoral portion was studied histologically using hematoxylin and eosin, alcian blue, toluidine blue, and Mason's trichrome. Pig cells and ...Continue Reading

References

Jan 1, 1989·The Journal of Bone and Joint Surgery. British Volume·S WakitaniK Ono
Jan 1, 1989·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·D A GrandeM Klein
Jan 1, 1987·Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery·H J Jaroma, V A Ritsilä
Jan 1, 1986·The Journal of Bone and Joint Surgery. British Volume·J E Aston, G Bentley
Dec 12, 1974·The New England Journal of Medicine·H J Mankin
Oct 6, 1994·The New England Journal of Medicine·M BrittbergL Peterson
Apr 1, 1993·The Journal of Bone and Joint Surgery. American Volume·F ShapiroM J Glimcher
May 1, 1996·Clinical Orthopaedics and Related Research·M BrittbergL Peterson
May 1, 1996·The Journal of Bone and Joint Surgery. American Volume·E B Hunziker, L C Rosenberg
Apr 1, 1996·Matrix Biology : Journal of the International Society for Matrix Biology·M MetsärantaE Vuorio
Jul 1, 1997·Clinical Orthopaedics and Related Research·C R ChuD Amiel
Nov 5, 1999·Clinical Orthopaedics and Related Research·M Brittberg
Nov 5, 1999·Clinical Orthopaedics and Related Research·R E SchreiberA Ratcliffe
Jan 1, 2000·The Journal of Bone and Joint Surgery. British Volume·J B RichardsonS Roberts
May 20, 2000·Clinical Orthopaedics and Related Research·L PetersonA Lindahl
Oct 11, 2002·The Journal of the American Academy of Orthopaedic Surgeons·Stephen A HuntOrrin H Sherman

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Citations

Sep 29, 2011·Tissue Engineering. Part a·Daniel J HueyKyriacos A Athanasiou
Dec 10, 2008·Tissue Engineering. Part B, Reviews·Christopher M Revell, Kyriacos A Athanasiou
Sep 10, 2010·Tissue Engineering. Part B, Reviews·Karym El SayedGundula Schulze-Tanzil
Jan 31, 2012·Veterinary and Comparative Orthopaedics and Traumatology : V.C.O.T·R M ThiedeM D Markel
Feb 2, 2013·Knee Surgery, Sports Traumatology, Arthroscopy : Official Journal of the ESSKA·Ki-Mo JangJoon Ho Wang
Nov 17, 2015·International Journal of Molecular Sciences·Alessandro R ZorziJoão B Miranda
Aug 7, 2009·Journal of Tissue Engineering and Regenerative Medicine·Najmuddin J GunjaKyriacos A Athanasiou
Nov 13, 2008·Journal of Tissue Engineering and Regenerative Medicine·Wan-Ju LiRocky S Tuan
Feb 9, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ming PeiBrandon M Boyce
Jun 2, 2015·Acta Biomaterialia·B ArziK A Athanasiou
Dec 23, 2008·Osteoarthritis and Cartilage·B J AhernT P Schaer
Jul 31, 2018·Journal of Tissue Engineering and Regenerative Medicine·Takumi TakahashiMasahiko Watanabe
Oct 22, 2014·Annals of Biomedical Engineering·Grayson D DuRaineKyriacos A Athanasiou
Nov 2, 2018·Archives of Orthopaedic and Trauma Surgery·Sebastian G WalterFrank A Schildberg
Sep 25, 2019·Stem Cell Research & Therapy·Baixiang ChengMin Zhang
Feb 23, 2020·Biochemistry Research International·Xiangbo MengXinluan Wang
Oct 1, 2007·Reumatología clinica·Isaac Manuel Fuentes-BoqueteFrancisco Javier Blanco García

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