Xenograft rejection of porcine islet-like cell clusters in normal, interferon-gamma, and interferon-gamma receptor deficient mice

Transplantation
J O SandbergOlle Korsgren

Abstract

The aim of the present study was to evaluate the role of the T-cell cytokine interferon (IFN)-gamma in mediating macrophage activation in xenograft rejection. For this purpose, fetal porcine islet-like cell cluster (ICC) transplants were placed under the renal capsule of normal mice and mice with a homozygous targeted disruption of the IFN-gamma or the IFN-gamma receptor gene. Some of the mice were continuously infused with cyclosporine (CsA, 12.4 mg/kg body weight/day) or CsA vehicle by subcutaneously implanted osmotic pumps. Histological evaluation of the xenografts was performed 6 or 12 days after transplantation. All animals, irrespective of recipient group, readily rejected the ICC xenograft, although the rejection process was slightly delayed in mice deficient in IFN-gamma. Analysis of the infiltrating cells within the xenograft in knockout mice revealed a pattern similar to that found in control animals. Six days after transplantation, there was an abundant infiltration of macrophages (Mac-1, F4/80, and major histocompatibility complex II markers) in the ICC grafts. Quite in contrast, there was only a low to moderate number of T cells (CD3 marker) present in the ICC grafts. Treatment with CsA had no effect on the rejecti...Continue Reading

References

Sep 1, 1989·The Journal of Experimental Medicine·R N PiersonH Auchincloss
Oct 1, 1994·Current Opinion in Immunology·A Dorling, R I Lechler
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Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R KamijoJ Vilcek

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Citations

Jul 17, 1999·Human Immunology·S BrouardJ P Soulillou
Feb 25, 2000·Allergy·G Tau, P Rothman
Dec 13, 2005·Transplantation·Shounan YiPhilip J O'Connell
Jul 18, 2002·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Xiao-Chun XuT Mohanakumar
Mar 12, 2004·Biochemical and Biophysical Research Communications·Jung-Sik KimChung-Gyu Park
Aug 18, 2000·The Journal of Surgical Research·T MakiS Ikeda
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·A FoxL C Harrison
Feb 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Shounan YiPhilip J O'Connell

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