Xenomonitoring of different filarial nematodes using single and multiplex PCR in mosquitoes from Assiut Governorate, Egypt

The Korean Journal of Parasitology
Ahmed Kamal DyabYasser Mokhtar

Abstract

Wuchereria bancrofti, Dirofilaria immitis, and Dirofilaria repens are filarial nematodes transmitted by mosquitoes belonging to Culex, Aedes, and Anopheles genera. Screening by vector dissection is a tiresome technique. We aimed to screen filarial parasites in their vectors by single and multiplex PCR and evaluate the usefulness of multiplex PCR as a rapid xenomonitoring and simultaneous differentiation tool, in area where 3 filarial parasites are coexisting. Female mosquitoes were collected from 7 localities in Assiut Governorate, were microscopically identified and divided into pools according to their species and collection site. Detection of W. bancrofti, D. immitis, and D. repens using single PCR was reached followed by multiplex PCR. Usefulness of multiplex PCR was evaluated by testing mosquito pools to know which genera and species are used by filarial parasites as a vector. An overall estimated rate of infection (ERI) in mosquitoes was 0.6%; the highest was Culex spp. (0.47%). W. bancrofti, D. immitis, and D. repens could be simultaneously and differentially detected in infected vectors by using multiplex PCR. Out of 100 mosquito pools, 8 were positive for W. bancrofti (ERI of 0.33%) and 3 pools each were positive for D...Continue Reading

Citations

Jul 16, 2016·The Korean Journal of Parasitology·Ahmed K DyabYasser Mokhtar
Oct 18, 2018·The Onderstepoort Journal of Veterinary Research·Peter NambalaChihiro Sugimoto
Dec 29, 2020·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mary M Cameron, Anita Ramesh

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Methods Mentioned

BETA
dissection
PCR
electrophoresis

Software Mentioned

Excel
SPSS

Related Concepts