Xenopus Cdc14 alpha/beta are localized to the nucleolus and centrosome and are required for embryonic cell division

BMC Cell Biology
Brett K KaiserPeter K Jackson

Abstract

The dual specificity phosphatase Cdc14 has been shown to be a critical regulator of late mitotic events in several eukaryotes, including S. cerevisiae, S. pombe. C. elegans and H. sapiens. However, Cdc14 homologs have clearly evolved to regulate distinct cellular processes and to respond to regulatory signals important for these processes. The human paralogs hCdc14A and B are the only vertebrate Cdc14 homologues studied to date, but their functions are not well understood. Therefore, it is of great interest to examine the function Cdc14 homologs in other vertebrate species. We identified two open reading frames from Xenopus laevis closely related to human Cdc14A, called XCdc14alpha and XCdc14beta, although no obvious paralog of the hCdc14B was found. To begin a functional characterization of Xcdc14alpha and XCdc14beta, we raised polyclonal antibodies against a conserved region. These antibodies stained both the nucleolus and centrosome in interphase Xenopus tissue culture cells, and the mitotic centrosomes. GFP-tagged version of XCdc14alpha localized to the nucleulus and GFP-XCdc14beta localized to the centrosome, although not exclusively. XCdc14alpha was also both meiotically and mitotically phosphorylated. Injection of antibo...Continue Reading

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Citations

May 19, 2005·Molecular and Cellular Biology·Hyekyung P ChoYisong Wang
Dec 24, 2010·Development·Aurélie ClémentKathleen L Gould
Jun 23, 2012·PloS One·Manuel Rosa-GarridoAlberto Gandarillas
Jul 1, 2017·Journal of Cell Science·Brendan L Powers, Mark C Hall
Jun 28, 2018·Biology Open·Leif R NeitzelEthan Lee
Oct 3, 2012·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Aurélie ClémentKathleen L Gould
Mar 2, 2006·Journal of Cell Science·Andrés Clemente-BlancoJaime Correa-Bordes
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Feb 13, 2007·Experimental Cell Research·Liliana KrasinskaNathalie Morin

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Methods Mentioned

BETA
x-ray crystallography
gel filtration

Software Mentioned

MEGABLAST
PSI
BLAST

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