Xenopus laevis Oocytes as a Model System for Studying the Interaction Between Asbestos Fibres and Cell Membranes

Toxicological Sciences : an Official Journal of the Society of Toxicology
Annalisa BernareggiGiuliano Zabucchi

Abstract

The mode of interaction of asbestos fibres with cell membranes is still debatable. One reason is the lack of a suitable and convenient cellular model to investigate the causes of asbestos toxicity. We studied the interaction of asbestos fibres with Xenopus laevis oocytes, using electrophysiological and morphological methods. Oocytes are large single cells, with a limited ability to endocytose molecular ligands; we therefore considered these cells to be a good model for investigating the nature of asbestos/membrane interactions. Electrophysiological recordings were performed to compare the passive electrical membrane properties, and those induced by applying positive or negative voltage steps, in untreated oocytes and those exposed to asbestos fibre suspensions. Ultrastructural analysis visualized in detail, any morphological changes of the surface membrane caused by the fibre treatment. Our results demonstrate that Amosite and Crocidolite-type asbestos fibres significantly modify the properties of the membrane, starting soon after exposure. Cells were routinely depolarized, their input resistance decreased, and the slow outward currents evoked by step depolarizations were dramatically enhanced. Reducing the availability of surf...Continue Reading

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Citations

Jan 11, 2018·International Journal of Environmental Research and Public Health·Violetta BorelliGiuliano Zabucchi
Sep 14, 2017·Scientific Reports·Daniele ZanellaGiovanni Bernardini
Aug 3, 2017·Environmental Science and Pollution Research International·Tingting HuoDongping Sun
Jun 1, 2019·Biochimica Et Biophysica Acta. Biomembranes·Daniele ZanellaGiovanni Bernardini

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