PMID: 8938732Jan 1, 1996Paper

Xenopus laevis oocytes contain endogenous large conductance Ca2(+)-activated K+ channels

Neuropharmacology
J D KrauseP H Reinhart

Abstract

Xenopus laevis oocytes have become a pre-eminent tool for studying cloned ion channels, primarily because they intrinsically express low levels of most types of ion channels. However, when these cells are used for single channel studies, it is essential to determine whether or not oocytes contain even low levels of endogenous ion channels with properties similar to the channel being investigated. We show here that X. laevis oocytes express endogenous large-conductance Ca2(+)-activated K+ channels with properties similar to mammalian isoforms of this channel. The endogenous channels exhibit a voltage-dependence of 12-14 mV per e-fold change in open probability (po), can be activated by micromolar Ca2+ concentrations, and have a single channel conductance of approximately 200 pS in symmetrical 110 mM K+ solutions. Patch clamp experiments indicate that this endogenous channel is present at low densities (approximately 1 channel/3000 microns2). If endogenous channel subunits can form functional tetramers with other exogenous potassium channel subunits, then they will give rise to the expression of a heterogeneous channel population. Therefore, studies involving the heterologous expression of large-conductance Ca2(+)-activated K+ ch...Continue Reading

References

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Citations

Dec 23, 2008·Cellular and Molecular Life Sciences : CMLS·J CuiU S Lee
Oct 13, 2010·Pflügers Archiv : European journal of physiology·Asser Nyander PoulsenDan Arne Klaerke
Mar 22, 2002·British Journal of Pharmacology·Sigrid WittmannStephen Daniels
Aug 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Xiang Qian, Karl L Magleby
Jul 27, 2005·The Journal of General Physiology·Tinatin I Brelidze, Karl L Magleby
Oct 19, 2011·Metallomics : Integrated Biometal Science·Paola GavazzoCarla Marchetti
Dec 6, 2002·Nature·Rebecca Piskorowski, Richard W Aldrich

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