Xenopus LIM motif-containing protein kinase, Xlimk1, is expressed in the developing head structure of the embryo

Developmental Dynamics : an Official Publication of the American Association of Anatomists
T TakahashiT Nakamura

Abstract

The LIM double zinc finger motif locates in several developmentally functioning and cytoskeletal proteins, and is considered to act as a specific motif for protein-protein interactions. LIM kinase (LIMK) is a novel protein kinase containing two LIM motifs at the N-terminal, the function of which has yet to be clearly defined. In this study, we cloned a cDNA encoding Xenopus counterpart of human LIMK1 gene by RT-PCR mediated cloning, and designated in Xlimk1. Xlimk1 is highly homologous to mammalian LIMK1 in each structural domain, particularly in LIM and protein kinase domains. In Northern blot analysis, two distinct Xlimk1 transcripts of 9.0 Kb and 3.7 Kb were present in early cleavage stages of the embryo. Both mRNA species were subsequently decreased at the gastrula stages. The 9.0 Kb of Xlimk1 mRNA again appeared in late neurula stage, then the expression level gradually increased in later stages of the embryo. Whole-mount in situ hybridization analysis showed the localization of Xlimk1 transcripts in the animal half of the blastula embryo. In post-neurula stages, specific signals for Xlimk1 were predominant in the anterior (head) region of the embryo, including developing brain, hyoid and branchial arches, and anlagen of s...Continue Reading

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Citations

Oct 12, 2000·Biochemical and Biophysical Research Communications·K OhashiK Mizuno
Feb 16, 2007·Gene Expression Patterns : GEP·Elisabeth B OttChristoph P Bagowski
Feb 4, 2006·Experimental Cell Research·Tomoyuki SumiToshikazu Nakamura
Apr 14, 2009·Developmental Biology·Jennifer C HockingSarah McFarlane
Jan 29, 2002·Biochemical and Biophysical Research Communications·Tomoyuki SumiToshikazu Nakamura
Feb 13, 2007·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Rebecca W Scott, Michael F Olson
May 8, 2001·The Journal of Biological Chemistry·T SumiT Nakamura
Aug 26, 1998·Biochemical and Biophysical Research Communications·H TakahashiT Nakamura
Jan 11, 2001·Developmental Biology·T TakahashiT Nakamura
Jan 11, 2002·Developmental Biology·Hisaaki TakahashiToshikazu Nakamura

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