Xenopus poly (A) binding protein maternal RNA is localized during oogenesis and associated with large complexes in blastula

Developmental Genetics
K E Schroeder, H J Yost

Abstract

Maternal mRNAs are synthesized during oogenesis and often stored for use during early embryogenesis, before the onset of zygotic transcription. The temporal and spatial regulation of maternal RNAs is likely to be crucial mechanism for the establishment of the body pattern. In the course of a study that identified a Xenopus maternal mRNA that is translationally regulated along the dorsoventral axis, several RNAs were found to behave anomalously in polysomal analysis and are further characterized here. As controls for polysome analysis, elF4E RNA and D7.1 RNA were equally translated in both dorsal and ventral cells, whereas the cell-cell signaling factor noggin RNA was not translated in either cell type. Maternal RNAs encoding poly (A) binding protein (PABP), Vg1 and Xcat-2 were associated with large complexes that, in contrast to polysomes, were not dissociated in magnesium-free buffer. Vg1 and Xcat-2 maternal mRNAs have been shown to be localized during oogenesis to the vegetal hemisphere of the oocyte [Rebagliati et al., 1985; Mosquera et al., 1993]. In situ hybridization analysis indicated that PABP RNA was also localized during oogenesis, to the animal hemisphere in stage VI oocytes. This suggests that association of materna...Continue Reading

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Citations

Jan 1, 1996·Developmental Genetics·M L King
Mar 4, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K L Mowry, C A Cote
Sep 3, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M L KingM Bubunenko
Oct 25, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Paaqua A GrantSally A Moody
Dec 17, 2004·Biology of the Cell·Mary Lou KingKimberly L Mowry
Apr 23, 2009·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Chanjae LeeJohn B Wallingford
Nov 8, 2002·Development·Todd T KrollPaul W Huber
Sep 3, 1999·Mechanisms of Development·H MacArthurM L King

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