Xenopus Xotx2 and Drosophila otd share similar activities in anterior patterning of the frog embryo

Development Genes and Evolution
Andrea Lunardi, R Vignali

Abstract

Despite the obvious anatomical differences between the fly and the vertebrate body plans, several genes involved in their development are largely conserved. In this work we provide evidence that overexpression of the Drosophila orthodenticle (otd) gene in Xenopus laevis has a similar effect to that of its homolog Xotx2. Injections of otd mRNA in whole embryos lead to posterior truncations and to induction of ectopic cement glands, similar to Xotx2 injections. In animal cap assays, otd, like Xotx2, is able to activate the cement gland marker XAG and to suppress the expression of the epidermal marker XK81. Finally, as assayed by Einsteck transplantation assays, otd, like Xotx2, is able to respecify a tail/trunk organizer to a head organizer. In this work we also show that Xotx2 and otd share molecular functions that regulate early regional specification of the Xenopus anterior neural plate. Gain-of-function experiment targeting low doses of either otd or Xotx2 mRNAs in the neural plate promote reduction of Xrx1 and Xbf1 expression domain; no changes are observed for the anterior mesodermal marker Xgsc, the dorsal diencephalic marker Xbh1, and the midbrain/hindbrain marker Xen2. otd/Xotx2 inhibition activity of Xrx1 and Xbf1 expre...Continue Reading

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Citations

Feb 17, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Kazunobu BabaSusumu Imaoka
Nov 1, 2012·Mechanisms of Development·Leonardo BeccariPaola Bovolenta
Nov 24, 2011·Experimental Neurobiology·Jaeho YoonJaebong Kim
Jul 19, 2012·PLoS Genetics·Kaylin A AdipietroHiroaki Matsunami
May 10, 2012·Ontogenez·E S Pshennikova, A S Voronina

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