Xenotransplantation of human stem cells into the chicken embryo.

Journal of Visualized Experiments : JoVE
Jean-Luc BoullandJoel C Glover

Abstract

The chicken embryo is a classical animal model for studying normal embryonic and fetal development and for xenotransplantation experiments to study the behavior of cells in a standardized in vivo environment. The main advantages of the chicken embryo include low cost, high accessibility, ease of surgical manipulation and lack of a fully developed immune system. Xenotransplantation into chicken embryos can provide valuable information about cell proliferation, differentiation and behavior, the responses of cells to signals in defined embryonic tissue niches, and tumorigenic potential. Transplanting cells into chicken embryos can also be a step towards transplantation experiments in other animal models. Recently the chicken embryo has been used to evaluate the neurogenic potential of human stem and progenitor cells following implantation into neural anlage. In this video we document the entire procedure for transplanting human stem cells into the developing central nervous system of the chicken embryo. The procedure starts with incubation of fertilized eggs until embryos of the desired age have developed. The eggshell is then opened, and the embryo contrasted by injecting dye between the embryo and the yolk. Small lesions are mad...Continue Reading

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Citations

Jun 20, 2013·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Mrinal JoelJoel C Glover
Apr 3, 2014·Journal of Cellular Biochemistry·Haijing ZhuJinlian Hua
Oct 4, 2015·The Journal of Pharmacology and Experimental Therapeutics·Sigrid BjørnstadRagnhild Elisabeth Paulsen
Oct 3, 2019·Journal of Neurophysiology·Hayley E SealKenna D Peusner
Oct 26, 2018·Frontiers in Genetics·Hicham Sid, Benjamin Schusser
Dec 29, 2020·Stem Cell Reports·Azimeh AkhlaghpourSeyedeh-Nafiseh Hassani

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Methods Mentioned

BETA
fluorescence activated cell sorting
dissection

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