Xiao-Xu-Ming Decoction Reduced Mitophagy Activation and Improved Mitochondrial Function in Cerebral Ischemia and Reperfusion Injury

Behavioural Neurology
Rui LanJi-Tao Wu

Abstract

We investigated whether Xiao-Xu-Ming decoction reduced mitophagy activation and kept mitochondrial function in cerebral ischemia-reperfusion injury. Rats were randomly divided into 5 groups: sham, ischemia and reperfusion (IR), IR plus XXMD (60 g/kg/day) (XXMD60), IR plus cyclosporin A (10 mg/kg/day) (CsA), and IR plus vehicle (Vehicle). Focal cerebral ischemia and reperfusion models were induced by middle cerebral artery occlusion (MCAO). Cerebral infarct areas were measured by triphenyl tetrazolium chloride staining. Cerebral ischemic injury was evaluated by hematoxylin and eosin staining (HE) and Nissl staining. Ultrastructural features of mitochondria and mitophagy in the penumbra of the ischemic cortex were observed by transmission electron microscopy. Mitophagy was detected by immunofluorescence labeled with LC3B and VDAC1. Autophagy lysosome formation was observed by immunofluorescence labeled with LC3B and Lamp1. The expression of LC3B, Beclin1, and Lamp1 was analyzed by Western blot. The rats subjected to MCAO showed worsened neurological score and cell ischemic damage. These were all significantly reversed by XXMD or CsA. Moreover, XXMD/CsA notably downregulated mitophagy and reduced the increase in LC3, Beclin1, and ...Continue Reading

References

Jul 1, 1996·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H HaraM A Moskowitz
Nov 5, 2004·Nature·Akiko KumaNoboru Mizushima
Jul 29, 2006·Autophagy·Sara Rodriguez-EnriquezJohn J Lemasters
Aug 12, 2006·The Journal of Biological Chemistry·Tomohiro YorimitsuDaniel J Klionsky
May 4, 2007·Archives of Biochemistry and Biophysics·Insil KimJohn J Lemasters
Jun 21, 2007·The Journal of Biological Chemistry·Serhiy PankivTerje Johansen
Jan 12, 2008·The American Journal of Pathology·Masato KoikeYasuo Uchiyama
Nov 26, 2008·Autophagy·Walter BalduiniGiuseppe Buonocore
Jan 26, 2010·Nature Cell Biology·Sven GeislerWolfdieter Springer
Oct 12, 2010·Autophagy·FengFeng TianKoji Abe
Apr 9, 2013·Neurochemical Research·Kangyong LiuXiaojiang Sun
Apr 16, 2013·Journal of Ethnopharmacology·Deng-Lei FuGuo-Qing Zheng
May 3, 2013·Neurotoxicity Research·Datang YuZhengfeng Zhang
Jan 21, 2014·Biochemical and Biophysical Research Communications·Qiang LiXiaojiang Sun
Jun 5, 2014·Nature·Alban Ordureau, J Wade Harper
Jun 19, 2014·Stroke; a Journal of Cerebral Circulation·Seung-Hoon BaekOk-Nam Bae
Jul 24, 2014·Toxicology Mechanisms and Methods·Xue WeiYingmei Zhang
Sep 18, 2014·CNS Neuroscience & Therapeutics·Ruo-Yang ShiJi-Ming Kong
Jul 30, 2015·Neuroscience Bulletin·Yang YuanZhong Chen
Aug 25, 2016·Protein & Cell·Yan-Cheng TangHu-Biao Chen

❮ Previous
Next ❯

Citations

Nov 9, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jinjing WuJun Ma
Jan 12, 2021·Frontiers in Neurology·Ziqi ShaoBo Bai
May 9, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Nannan LiuGuanhua Du
Jul 19, 2021·Molecular Neurobiology·Mimi WuZhengliang Ma
Oct 9, 2021·Current Pharmaceutical Design·Li-Ping YuXing-Xin Yang
Jan 19, 2022·Journal of Cellular and Molecular Medicine·Heyan TianJinwen Ge

❮ Previous
Next ❯

Methods Mentioned

BETA
MDA
electrophoresis
electron microscopy

Software Mentioned

Quantity One
SPSS

Related Concepts

Related Feeds

Brain Ischemia

Brain ischemia is a condition in which there is insufficient blood flow to the brain to meet metabolic demand. Discover the latest research on brain ischemia here.

Autophagy & Model Organisms

Autophagy is a cellular process that allows degradation by the lysosome of cytoplasmic components such as proteins or organelles. Here is the latest research on autophagy & model organisms