XIAP is related to the chemoresistance and inhibited its expression by RNA interference sensitize pancreatic carcinoma cells to chemotherapeutics

Pancreas
Yixiong LiJindong Li

Abstract

To explore the exact role does the x-linked inhibitor of apoptosis (XIAP) play in chemoresistance of pancreatic carcinoma cell and the cell sensitivity to chemotherapeutic drugs changed after XIAP is inhibited by RNA interference (RNAi). Pancreatic carcinoma cell line SW1990 was exposed to 5-fluorouracil (5-fu) with the concentrations of 1.0 and 10 mug/mL to increase the expression of XIAP. Then 4 RNAi plasmid vectors against XIAP were designed and constructed, then transfected into SW1990. Repressive effect was evaluated by reverse transcriptase polymerase chain reaction and Western blot; 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and flow cytometry were performed to determine cell sensitivity to 5-fu and gemcitabine; furthermore, apoptosis is confirmed by Hoechst 33258 stain. XIAP of SW1990 can be up-regulated with the chemoresistance increasing 1.5- and 4-fold after 10 and 30 days induced by 5-fu. Two of the 4 vectors can inhibit the expression of XIAP protein more than 60%. The cells apoptosis index induced by 5-fu and gemcitabine increased greatly after XIAP is inhibited by the RNAi plasmid vectors. XIAP is one of the most important factors in the pancreatic carcinoma chemoresistance, and inhibition of XI...Continue Reading

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Citations

Oct 14, 2011·Future Oncology·Ronit Elhasid, Sarit Larisch
Jun 28, 2012·World Journal of Gastroenterology : WJG·Chun JiangYi-Xiong Li
Oct 15, 2013·Pathology Oncology Research : POR·Mehdi ManoochehriBahram Kazemi
Sep 26, 2007·Expert Review of Anticancer Therapy·Simone Fulda
Apr 17, 2013·Expert Opinion on Investigational Drugs·Lakshmikanth KatragaddaGautam Borthakur
Apr 4, 2008·Phytotherapy Research : PTR·Sin Ting LauPo Sing Leung
Jun 3, 2017·Infectious Agents and Cancer·Katharina BeyerWolfram von Bernstorff
Nov 14, 2018·Pathology Oncology Research : POR·Xi-Hu YangQin-Gang Hu
Jul 26, 2008·Molecular Cancer·Rainer HamacherGünter Schneider

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis