XPS and AFM characterization of the enzyme glucose oxidase immobilized on SiO(2) surfaces

Langmuir : the ACS Journal of Surfaces and Colloids
Sebania LibertinoManuela Fichera

Abstract

A process to immobilize the enzyme glucose oxidase on SiO2 surfaces for the realization of integrated microbiosensors was developed. The sample characterization was performed by monitoring, step by step, oxide activation, silanization, linker molecule (glutaraldehyde) deposition, and enzyme immobilization by means of XPS, AFM, and contact angle measurements. The control of the environment during the procedure, to prevent silane polymerization, and the use of oxide activation to obtain a uniform enzyme layer are issues of crucial importance. The correct protocol application gives a uniform layer of the linker molecule and the maximum sample surface coverage. This result is fundamental for maximizing the enzyme bonding sites on the sample surface and achieving the maximum surface coverage. Thin SiO2 layers thermally grown on a Si substrate were used. The XPS Si 2p signal of the substrate was monitored during immobilization. Such a signal is not completely shielded by the thin oxide layer and it is fully suppressed after the completion of the whole protocol. A power spectral density analysis on the AFM measurements showed the crucial role of both the oxide activation and the intermediate steps (silanization and linker molecule dep...Continue Reading

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Citations

Sep 3, 2010·Applied Microbiology and Biotechnology·Raushan Kumar SinghJung-Kul Lee
Apr 24, 2013·Enzyme and Microbial Technology·Nesrine AissaouiJean-François Lambert
Sep 14, 2010·Nanotechnology·Ellen BlinkaJohn X J Zhang
Mar 10, 2012·International Journal of Nanomedicine·Tuck-yun CheangShen-ming Wang
Oct 6, 2015·Nano Letters·Xing MaSamuel Sánchez
Mar 3, 2009·Biosensors & Bioelectronics·Jog RajDamien W M Arrigan
Nov 7, 2013·Applied Microbiology and Biotechnology·Raushan Kumar SinghJung-Kul Lee
Oct 22, 2016·Journal of Food Science and Technology·Ruchi AgrawalA K Verma
Dec 10, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Xing ZhuWantai Yang
Jan 1, 2009·Sensors·Sebania LibertinoSalvatore Lombardo
Oct 24, 2013·Chemical Communications : Chem Comm·Yong-Kyun SimChul-Ho Jun
Jan 1, 2013·Cancer Nanotechnology·Chandrababu RejeethSoundarapandian Kannan
Apr 2, 2010·ACS Applied Materials & Interfaces·Amal KasryTrevor Dale
Mar 26, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Giuseppe ArrabitoBruno Pignataro
Dec 15, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Elisabeth BriandSofia Svedhem
Aug 28, 2014·ACS Applied Materials & Interfaces·Giorgi ShtenbergEster Segal
Nov 22, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Yue LiuTao He
Aug 8, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Walid-Madhat MuniefSven Ingebrandt

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