XpsE oligomerization triggered by ATP binding, not hydrolysis, leads to its association with XpsL

The EMBO Journal
Sheng-Jie ShiueNien-Tai Hu

Abstract

GspE belongs to a secretion NTPase superfamily, members of which are involved in type II/IV secretion, type IV pilus biogenesis and DNA transport in conjugation or natural transformation. Predicted to be a cytoplasmic protein, GspE has nonetheless been shown to be membrane-associated by interacting with the N-terminal cytoplasmic domain of GspL. By taking biochemical and genetic approaches, we observed that ATP binding triggers oligomerization of Xanthomonas campestris XpsE (a GspE homolog) as well as its association with the N-terminal domain of XpsL (a GspL homolog). While isolated XpsE exhibits very low intrinsic ATPase activity, association with XpsL appears to stimulate ATP hydrolysis. Mutation at a conserved lysine residue in the XpsE Walker A motif causes reduction in its ATPase activity without significantly influencing its interaction with XpsL, congruent with the notion that XpsE-XpsL association precedes ATP hydrolysis. For the first time, functional significance of ATP binding to GspE in type II secretion system is clearly demonstrated. The implications may also be applicable to type IV pilus biogenesis.

References

Mar 1, 1992·Molecular Microbiology·S KamounC I Kado
Apr 1, 1994·Molecular Microbiology·O Possot, A P Pugsley
Feb 2, 1999·The EMBO Journal·J TurnerM O'Donnell
Jul 8, 1999·Proceedings of the National Academy of Sciences of the United States of America·N NouwenA P Pugsley
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·P J PlanetD H Figurski
Apr 20, 2001·Molecular Microbiology·M Sandkvist
May 3, 2003·The EMBO Journal·Savvas N SavvidesGabriel Waksman
May 9, 2003·The Journal of Biological Chemistry·Fumihiro Motojima, Masasuke Yoshida
Oct 15, 2003·Journal of Molecular Biology·Mark A RobienWim G J Hol
Nov 18, 2004·Biochimica Et Biophysica Acta·Alain Filloux
Dec 17, 2004·Journal of Bacteriology·Jodi L Camberg, Maria Sandkvist
May 4, 2005·The Journal of Biological Chemistry·Lynette J CrowtherMichael S Donnenberg
Sep 1, 2005·The Journal of Biological Chemistry·Mohamed ChamiNicolas Bayan

❮ Previous
Next ❯

Citations

Apr 2, 2013·Research in Microbiology·S Peter Howard
Mar 7, 2012·The Journal of Biological Chemistry·Lay-Sun MaErh-Min Lai
Mar 14, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Badreddine DouziRomé Voulhoux
Jan 29, 2008·Journal of Bacteriology·Vladimir JakovljevicLotte Søgaard-Andersen
Aug 21, 2010·Future Microbiology·Melissa AyersLori L Burrows
Dec 13, 2006·The EMBO Journal·Jodi L CambergMaria Sandkvist
Feb 27, 2008·Molecular Microbiology·Nicholas Renzette, Steven J Sandler
Jan 7, 2014·Biochimica Et Biophysica Acta·Mangayarkarasi Nivaskumar, Olivera Francetic
Sep 4, 2008·Biochemical and Biophysical Research Communications·Jye-Lin HsuHwan-You Chang
Mar 21, 2015·FEMS Microbiology Reviews·Jamie-Lee Berry, Vladimir Pelicic
Sep 1, 2016·Nature Communications·Yu-Chuan WangShan-Ho Chou
Dec 2, 2017·Molecular Microbiology·Paushali ChaudhurySonja-Verena Albers
May 10, 2017·Molecular Microbiology·Jenny-Lee ThomassinOlivera Francetic
Nov 16, 2019·Nature Communications·Matthew McCallumP Lynne Howell
Dec 8, 2020·Molecular Microbiology·Souvik NaskarHarry H Low
Mar 5, 2021·Bio-protocol·Amita KaundalKirankumar S Mysore
Aug 17, 2021·PLoS Pathogens·Edgar E LlontopChuck S Farah

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.