Xuebijing Injection () and Resolvin D1 Synergize Regulate Leukocyte Adhesion and Improve Survival Rate in Mice with Sepsis-Induced Lung Injury

Chinese Journal of Integrative Medicine
Shu-Kun ZhangXi-Mo Wang

Abstract

To investigate the effect of combined application of Xuebijing Injection ( , XBJ) and resolvin D1 (RvD1) on survival rate and the underlying mechanisms in mice with sepsisinduced lung injury. The cecal ligation and puncture (CLP) method was used to develop a mouse sepsis model. Specific pathogen free male C57BL/6 mice were randomly divided into 5 groups (n=20 each): sham, CLP, CLP+XBJ, CLP+RvD1 and CLP+XBJ+RvD1. After surgery, mice in the CLP+XBJ, CLP+RvD1 and CLP+XBJ+RvD1 groups were given XBJ (25 μL/g body weight), RvD1 (10 ng/g body weight), and their combination (the same dose of XBJ and RvD1), respectively. In each group, 12 mice were used to observe 1-week survival rate, while the rest were executed at 12 h. Whole blood was collected for flow cytometric analysis of leukocyte adhesion molecules CD18, lung tissues were harvested for observing pathological changes, and testing the activity of myeloperoxidase (MPO) and the expression of intercellular cell adhesion molecule 1 (ICAM-1). Compared with the CLP group, the histopathological damage of the lung tissues was mitigated, MPO activity was decreased in the CLP+XBJ and CLP+RvD1 groups (P<0.05). In addition, the 1-week survival rate was improved, proportion of CD18-expressin...Continue Reading

References

Jan 1, 1994·Annual Review of Medicine·M P BevilacquaO Cecconi
Feb 29, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·A MatsukawaS L Kunkel
Aug 18, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·X GaoA B Malik
Mar 7, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ning XuAsrar B Malik
Apr 18, 2003·The New England Journal of Medicine·Greg S MartinMarc Moss
Oct 18, 2003·Cytokine & Growth Factor Reviews·Richard B GoodmanMichael A Matthay
Jan 10, 2009·Nature Protocols·Daniel RittirschPeter A Ward
Feb 25, 2011·Current Opinion in Critical Care·Thierry Gustot
May 26, 2011·Critical Care Medicine·Faraz M NorozianBalagangadhar R Totapally
Feb 6, 2013·European Journal of Pharmacology·Yongzhi WangHenrik Thorlacius
Feb 25, 2014·The Journal of Surgical Research·Ching-Jen WangHsueh-Wen Chang
Feb 27, 2015·Saudi Medical Journal·Si-Yuan HouYong-Feng Tan

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Citations

Dec 16, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Wenjing LiaoHe Sun
May 22, 2021·Nanoscale Research Letters·Wenjie ZhangTianmin Li

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