Xylan synthesized by Irregular Xylem 14 (IRX14) maintains the structure of seed coat mucilage in Arabidopsis

Journal of Experimental Botany
Ruibo HuYingzhen Kong

Abstract

During differentiation, the Arabidopsis seed coat epidermal cells synthesize and secrete large quantities of pectinaceous mucilage into the apoplast, which is then released to encapsulate the seed upon imbibition. In this study, we showed that mutation in Irregular Xylem 14 (IRX14) led to a mucilage cohesiveness defect due to a reduced xylan content. Expression of IRX14 was detected specifically in the seed coat epidermal cells, reaching peak expression at 13 days post-anthesis (DPA) when the accumulation of mucilage polysaccharides has ceased. Sectioning of the irx14-1 seed coat revealed no visible structural change in mucilage secretory cell morphology. Although the total amount of mucilage was comparable with the wild type (WT), the partition between water-soluble and adherent layers was significantly altered in irx14-1, with redistribution from the adherent layer to the water-soluble layer. The monosaccharide composition analysis revealed that xylose content was significantly reduced in irx14-1 water-soluble and adherent mucilage compared with the WT. The macromolecular characteristics of the water-soluble mucilage were modified in irx14-1 with a loss of the larger polymeric components. In accordance, glycome profiling and ...Continue Reading

References

Dec 1, 1969·Analytical Biochemistry·D M Updegraff
Mar 4, 2000·Plant Physiology·T L WesternG W Haughn
Jul 8, 2000·The Plant Journal : for Cell and Molecular Biology·J B WindsorA M Lloyd
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 4, 2003·Analytical Biochemistry·Alexander Laurentin, Christine A Edwards
Apr 5, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Lesley McCartneyJ Paul Knox
Oct 20, 2007·The Plant Journal : for Cell and Molecular Biology·David M BrownSimon R Turner
Nov 5, 2008·The Plant Journal : for Cell and Molecular Biology·David M BrownSimon R Turner
Mar 3, 2010·Annual Review of Plant Biology·Henrik Vibe Scheller, Peter Ulvskov
Apr 7, 2010·Plant Physiology·Sivakumar PattathilMichael G Hahn
Apr 14, 2010·Proceedings of the National Academy of Sciences of the United States of America·Brandon H LeRobert B Goldberg
May 28, 2010·Plant Signaling & Behavior·Andrej A ArsovskiTamara L Western
Dec 18, 2010·The Journal of Physical Chemistry. B·Daryl J DagelSteve Smith
Sep 3, 2011·The Plant Journal : for Cell and Molecular Biology·Smadar Harpaz-SaadJoseph J Kieber
Nov 8, 2011·Plant Signaling & Behavior·Venugopal MenduSeth DeBolt
May 29, 2012·Frontiers in Plant Science·Azeddine DriouichOlivier Lerouxel
Apr 1, 2014·Current Opinion in Biotechnology·Emilie A Rennie, Henrik Vibe Scheller
Feb 7, 2015·International Journal of Molecular Sciences·Cătălin VoiniciucBjörn Usadel
May 13, 2015·Carbohydrate Polymers·Liang LiKarim Mazeau
May 23, 2015·Trends in Plant Science·Edith FrancozChristophe Dunand
Jun 5, 2015·The Plant Journal : for Cell and Molecular Biology·Jenny C MortimerPaul Dupree

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Citations

Jul 5, 2016·Frontiers in Plant Science·Cătălin VoiniciucBjörn Usadel
Aug 16, 2016·Frontiers in Plant Science·Jonathan S GriffithsHelen M North
Jan 17, 2017·Scientific Reports·Xianhai ZhaoAi-Min Wu
Apr 12, 2020·Journal of Experimental Botany·Yiping WangRuibo Hu
Dec 14, 2018·Frontiers in Plant Science·Ringo van WijkTeun Munnik
Jul 12, 2019·Frontiers in Plant Science·Fabien MiartKarine Pageau
Jul 18, 2020·Scientific Reports·Jana L PhanRachel A Burton
Feb 11, 2021·Plant Biotechnology Journal·Yanrong LiuWanjun Zhang
Apr 2, 2021·Plant Physiology·Susana Saez-AguayoAriel Orellana
Apr 10, 2021·Comprehensive Reviews in Food Science and Food Safety·Yan LiuSteve W Cui
Apr 1, 2021·The Plant Journal : for Cell and Molecular Biology·Sukhita SathitnaithamSupachai Vuttipongchaikij

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