Abstract
Microbial xylanases that are thermostable, active at alkaline pH and cellulase-free are generally preferred for biobleaching of paper pulp. We screened obligate and facultative marine fungi for xylanase activity with these desirable traits. Several fungal isolates obtained from marine habitats showed alkaline xylanase activity. The crude enzyme from NIOCC isolate 3 (Aspergillus niger), with high xylanase activity, cellulase-free and unique properties containing 580 U l(-1) xylanase, could bring about bleaching of sugarcane bagasse pulp by a 60 min treatment at 55 degrees C, resulting in a decrease of ten kappa numbers and a 30% reduction in consumption of chlorine during bleaching. The culture filtrate showed peaks of xylanase activity at pH 3.5 and pH 8.5. When assayed at pH 3.5, optimum activity was detected at 50 degrees C, with a second peak of activity at 90 degrees C. When assayed at pH 8.5, optimum activity was seen at 80 degrees C. The crude enzyme was thermostable at 55 degrees C for at least 4 h and retained about 60% activity. Gel filtration of the 50-80% ammonium sulphate-precipitated fraction of the crude culture filtrate separated into two peaks of xylanase with specific activities of 393 and 2,457 U (mg protein)(...Continue Reading
References
Jul 1, 1979·American Journal of Public Health·V J CabelliP W Haberman
Jul 1, 1993·Journal of Biotechnology·K E Eriksson
Mar 1, 1994·Applied Biochemistry and Biotechnology·M Costa-FerreiraJ C Duarte
Jul 1, 1993·Applied and Environmental Microbiology·S NakamuraK Horikoshi
Sep 3, 1998·Applied and Environmental Microbiology·A Gessesse
May 1, 1999·Applied and Environmental Microbiology·C RaghukumarC A Reddy
Nov 1, 1986·Applied and Environmental Microbiology·T D Leathers
May 1, 1993·Applied and Environmental Microbiology·P MoralesA Flors
Sep 1, 1996·Applied and Environmental Microbiology·M K KangY H Rhee
Apr 1, 1985·Biotechnology and Bioengineering·M M FrederickP J Reilly
Feb 5, 1990·Biotechnology and Bioengineering·S R BiswasG Nanda
Oct 20, 1991·Biotechnology and Bioengineering·D C Smith, T M Wood
Mar 1, 1993·World Journal of Microbiology & Biotechnology·S NakamuraK Horikoshi
Citations
Jan 22, 2010·Bioprocess and Biosystems Engineering·M MichelinM L T M Polizeli
Sep 1, 2005·Journal of Industrial Microbiology & Biotechnology·C Mohandass, Chandralata Raghukumar
Jan 23, 2008·Journal of Industrial Microbiology & Biotechnology·Tanmay DuttaSanjay Ghosh
Dec 5, 2012·World Journal of Microbiology & Biotechnology·Jeremy Martin O Torres, Thomas Edison E Dela Cruz
Sep 4, 2009·Applied Biochemistry and Biotechnology·Dhananjay SorenAnil K Ghosh
May 28, 2005·Biotechnology Advances·Maria Gavrilescu, Yusuf Chisti
Jul 16, 2010·Marine Drugs·Chen Zhang, Se-Kwon Kim
Jun 15, 2011·Bioscience, Biotechnology, and Biochemistry·Parichart KhonzueLily Eurwilaichitr
May 2, 2014·Marine Genomics·Erica M HartmannJean Armengaud
Jan 19, 2016·Applied Biochemistry and Biotechnology·Sandesh PanthiJin Cheol Yoo
Aug 30, 2008·Bioresource Technology·S SavithaK Swaminathan
Jan 30, 2007·Letters in Applied Microbiology·T DuttaS Ghosh
Aug 21, 2013·Journal of Applied Microbiology·S KamatW N Gade
Mar 25, 2016·AMB Express·Juliana A Dos SantosLara D Sette
Dec 13, 2005·Bioresource Technology·Suchita Ninawe, Ramesh Chander Kuhad
Apr 29, 2015·Frontiers in Microbiology·Rafaella C Bonugli-SantosLara D Sette
Feb 18, 2009·FEMS Microbiology Letters·Dawn Elizabeth StephensKugen Permaul
Aug 8, 2006·Biotechnology Journal·Assunta GiordanoAntonio Trincone
Sep 29, 2017·3 Biotech·G RamanjaneyuluB Rajasekhar Reddy
Jul 28, 2018·Frontiers in Microbiology·Larissa BalabanovaLiudmila Tekutyeva
Feb 14, 2006·Acta Biochimica Et Biophysica Sinica·Yun-Hua HouHui-Yuan Zhu
Oct 2, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chanika OuephanitWarawut Chulalaksananukul
Jan 1, 2013·3 Biotech·Sushil NagarVijay Kumar Gupta
Aug 2, 2017·Journal of Basic Microbiology·Melih N KorkmazAtaç Uzel
Jun 3, 2021·Scientific Reports·Qingqing PengXi Yu