Xylose as preferred substrate for sarcosine production by recombinant Corynebacterium glutamicum

Bioresource Technology
Melanie MindtVolker F Wendisch

Abstract

The aim of this work was to study the fermentative production of the N-methylated amino acid sarcosine by C. glutamicum. Characterization of the imine reductase DpkA from Pseudomonas putida revealed that it catalyses N-methylamination of glyoxylate to sarcosine. Heterologous expression of dpkA in a C. glutamicum strain engineered for glyoxylate overproduction enabled fermentative production of sarcosine from sugars and monomethylamine. Glucose-based fermentation reached sarcosine production titers of 2.4 ± 0.1 g L-1. Sarcosine production based on the second generation feedstocks xylose and arabinose led to higher product titers of 2.7 ± 0.1 g L-1 and 3.4 ± 0.3 g L-1, respectively, than glucose-based production. Optimization of production conditions with xylose and potassium acetate blends increased sarcosine titers to 8.7 ± 0.2 g L-1 with a yield of 0.25 g g-1. This is the first example in which a C. glutamicum process using lignocellulosic pentoses is superior to glucose-based production.

Citations

Mar 15, 2020·Biotechnology Journal·Melanie MindtVolker F Wendisch
Jan 31, 2020·Frontiers in Bioengineering and Biotechnology·Xiao-Yu WuBang-Ce Ye
Feb 6, 2021·Critical Reviews in Biotechnology·Hui LiuLiming Liu
Jan 5, 2021·Frontiers in Bioengineering and Biotechnology·Bin ZhangXiao-Yu Wu
Jun 12, 2020·Microorganisms·Tatjana WalterVolker F Wendisch
Apr 4, 2019·Metabolic Engineering·Volker F Wendisch
Oct 19, 2021·Frontiers in Bioengineering and Biotechnology·Arthur BurgardtVolker F Wendisch

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