Yaba tumor poxvirus synthesis in vitro. I. Cytopathological, histochemical, and immunofluorescent studies.

Journal of Bacteriology
D S YohnJ T Grace

Abstract

Yohn, David S. (Roswell Park Memorial Institute, Buffalo, N.Y.), Victoria A. Haendiges, and James T. Grace, Jr. Yaba tumor poxvirus synthesis in vitro. I. Cytopathological, histochemical, and immunofluorescent studies. J. Bacteriol. 91:1977-1985. 1966.-Yaba tumor poxvirus synthesis in BSC-1 cell culture was followed sequentially with light microscopy, immunofluorescent microscopy, and various histochemical stains. The first evidence of infection was detected at 24 hr when nucleoli became hypertrophic, reflecting enhanced ribonucleic acid (RNA) synthesis. At 36 hr, deoxyribonucleic acid synthesis was detected in the cytoplasm. This was immediately followed by or associated with antigen synthesis at paranuclear sites and enhanced RNA synthesis in the cytoplasm. Cytoplasmic inclusions were readily apparent at 4 days in initially infected cells. Contiguous spread of virus was judged to have occurred around the third day of infection. The time required to complete the synthetic cycle from time of infection to production of infectious progeny was estimated to be of the order of 60 hr. This cycle is 6 to 10 times longer than for vaccinia virus. By light microscopy, cytopathic effects (CPE) were detectable in 5 days in heavily infected...Continue Reading

References

May 1, 1966·Journal of Bacteriology·D S YohnE De Harven
Jul 19, 1958·Nature·W G BEARCROFT, M F JAMIESON
Mar 1, 1964·Journal of Molecular Biology·N P SALZMANE D SEBRING
May 30, 1964·Nature·D S YOHNV A HAENDIGES
Jun 1, 1965·Experimental and Molecular Pathology·A L ROQUEP COULTER

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Citations

Dec 1, 1971·Japanese Journal of Medical Science & Biology·T Yuasa, I Tagaya
Jul 3, 1969·Annals of the New York Academy of Sciences·G D Hsiung
Feb 1, 1970·Journal of Virology·R G Olsen, D S Yohn
Dec 1, 1973·Journal of Virology·H Rouhandeh, M L Rouhandeh
May 1, 1966·Journal of Bacteriology·D S YohnE De Harven

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