PMID: 8600568Nov 1, 1995Paper

YAC analysis and minimal tiling path construction for chromosome 21q

Somatic Cell and Molecular Genetics
K GardinerD Patterson

Abstract

We have undertaken a detailed analysis of several hundred YACs from widely available YAC libraries which map to human chromosome 21 with the goal of improving the physical map of chromosome 21 and determining the feasibility of producing a minimal tiling path of well characterized, stable, non-chimeric YACs spanning the long arm of the chromosome (21q). We report information on over 500 YACs known to contain STS from 21q including information on size, stability, chimerism, marker content, and NotI restriction sites. YACs derive from the CEPH and St. Louis YAC libraries, and STSs include the set of 198 markers originally used do assemble a YAC contig of 21q, as well as additional anonymous probes and gene markers. This information has assisted in refinements of STS order, has defined a region of general instability in 2lq22.3, has identified an increased number of NotI restriction sites, and has defined cryptic gaps, particularly in 2lq2l, for which few or no markers are available. These results have allowed us to develop and assess a minimal tiling path of overlapping YACs consisting of 59 YACs (and two PI clones), largely non chimeric, stable, and of verified STS content. They total 30 mb of non-overlapping DNA, and contain al...Continue Reading

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Citations

Nov 1, 1995·Somatic Cell and Molecular Genetics·S L GrawD Patterson
May 1, 1996·Somatic Cell and Molecular Genetics·D E CabinR H Reeves
Mar 1, 1997·Somatic Cell and Molecular Genetics·L VillardK Gardiner
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·J YuF T Kao
Jun 10, 2003·American Journal of Medical Genetics. Part a·Hilary Tinkel-VernonLeonard Sciorra
Jan 11, 2005·Nature Reviews. Genetics·David Patterson, Alberto C S Costa
Jun 1, 2000·Nature·M HattoriUNKNOWN Chromosome 21 mapping and sequencing consortium
Sep 29, 1998·Genome Research·D E CabinR H Reeves

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