Abstract
We have undertaken a detailed analysis of several hundred YACs from widely available YAC libraries which map to human chromosome 21 with the goal of improving the physical map of chromosome 21 and determining the feasibility of producing a minimal tiling path of well characterized, stable, non-chimeric YACs spanning the long arm of the chromosome (21q). We report information on over 500 YACs known to contain STS from 21q including information on size, stability, chimerism, marker content, and NotI restriction sites. YACs derive from the CEPH and St. Louis YAC libraries, and STSs include the set of 198 markers originally used do assemble a YAC contig of 21q, as well as additional anonymous probes and gene markers. This information has assisted in refinements of STS order, has defined a region of general instability in 2lq22.3, has identified an increased number of NotI restriction sites, and has defined cryptic gaps, particularly in 2lq2l, for which few or no markers are available. These results have allowed us to develop and assess a minimal tiling path of overlapping YACs consisting of 59 YACs (and two PI clones), largely non chimeric, stable, and of verified STS content. They total 30 mb of non-overlapping DNA, and contain al...Continue Reading
References
Jun 1, 1992·Nature Genetics·I M ChumakovE Barillot
Oct 1, 1992·Nature·I ChumakovL Grinas
Sep 18, 1992·Cell·C Bellanné-ChantelotG Lucotte
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·G UzéK E Mogensen
Dec 6, 1991·Science·L J Ferrin, R D Camerini-Otero
Jan 1, 1991·Genomics·M BurmeisterD R Cox
May 1, 1991·Genomics·J Z GaoH Drabkin
Jun 25, 1991·Nucleic Acids Research·J NasirS D Brown
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H M AlbertsenD Cohen
Oct 1, 1990·Genomics·T Imai, M V Olson
Nov 1, 1989·Genomics·B TraskG van den Engh
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D PattersonJ F Gusella
May 15, 1987·Science·D T BurkeM V Olson
Apr 10, 1995·Genomics·P PahlD Patterson
May 1, 1995·Genomics·H XuS M Weissman
Mar 1, 1995·Cancer Genetics and Cytogenetics·Y PekarskyK Gardiner
Jan 1, 1995·Cytogenetics and Cell Genetics
Sep 1, 1994·Genomics·J F ChengY Zhu
Oct 1, 1994·Human Molecular Genetics·N PatilD R Cox
Nov 1, 1994·Genomics·G PalmieriR Reinbold
Aug 1, 1994·Mammalian Genome : Official Journal of the International Mammalian Genome Society·A CiccodicolaB Molini
Aug 1, 1994·Genomics·D G NegorevH C Riethman
May 1, 1994·Human Molecular Genetics·D NizetićC Lopes
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·W M Barnes
Jan 1, 1994·Nature Genetics·P A IoannouP J de Jong
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·N S ShepherdN Sternberg
Feb 1, 1994·Genomics·M C Dufresne-ZachariaJ M Delabar
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·S ChengR Higuchi
Dec 1, 1993·Genomics·J M DelabarD Patterson
Aug 1, 1993·Nature Genetics·H IchikawaM Ohki
Nov 20, 1995·Genomics·K GardinerJ F Cheng
Citations
Nov 1, 1995·Somatic Cell and Molecular Genetics·S L GrawD Patterson
May 1, 1996·Somatic Cell and Molecular Genetics·D E CabinR H Reeves
Mar 1, 1997·Somatic Cell and Molecular Genetics·L VillardK Gardiner
Aug 3, 2001·Gene·S G KurdyukovE D Sverdlov
Jul 18, 1997·Gene·A De SarioG Bernardi
Feb 14, 1998·Gene·K Gardiner
Nov 26, 1999·Gene·M RuaultA De Sario
Jun 24, 1997·Proceedings of the National Academy of Sciences of the United States of America·J YuF T Kao
Nov 24, 1999·Genome Research·S Y WangC van Broeckhoven
Jul 1, 1997·Genomics·R OrtiJ M Delabar
Aug 17, 1999·Nature·S BowmanB G Barrell
Jun 10, 2003·American Journal of Medical Genetics. Part a·Hilary Tinkel-VernonLeonard Sciorra
Jan 11, 2005·Nature Reviews. Genetics·David Patterson, Alberto C S Costa
Jun 1, 2000·Nature·M HattoriUNKNOWN Chromosome 21 mapping and sequencing consortium
Sep 29, 1998·Genome Research·D E CabinR H Reeves