DOI: 10.1101/495499Dec 13, 2018Paper

YAP-independent mechanotransduction drives breast cancer progression

BioRxiv : the Preprint Server for Biology
Joanna Y. LeeOvijit Chaudhuri

Abstract

Increased tissue stiffness is a driver of breast cancer progression. The transcriptional regulator YAP is considered a universal mechanotransducer, based largely on 2D culture studies. However, the role of YAP during in vivo breast cancer remains unclear. Here, we find that mechanotransduction occurs independently of YAP in breast cancer patient samples and mechanically tunable 3D cultures. Mechanistically, the lack of YAP activity in 3D culture and in vivo is associated with the absence of stress fibers and an order of magnitude decrease in nuclear cross-sectional area relative to 2D culture. This work highlights the context-dependent role of YAP in mechanotransduction, and establishes that YAP does not mediate mechanotransduction in breast cancer.

Related Concepts

Malignant Neoplasm of Breast
Cell Culture Techniques
Fiber
Laboratory Culture
Three-dimensional
Cross-section
Mechanotransduction, Cellular
Transcriptional Regulation
In Vivo
Yorkie protein, Drosophila

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