Yeast adaptor protein, Nbp2p, is conserved regulator of fungal Ptc1p phosphatases and is involved in multiple signaling pathways.

The Journal of Biological Chemistry
Karen StangerAlan R Davidson

Abstract

Nbp2p is an Src homology 3 (SH3) domain-containing yeast protein that is involved in a variety of cellular processes. This small adaptor protein binds to a number of different proteins through its SH3 domain, and a region N-terminal to the SH3 domain binds to the protein phosphatase, Ptc1p. Despite its involvement in a large number of physical and genetic interactions, the only well characterized function of Nbp2p is to recruit Ptc1p to the high osmolarity glycerol pathway, which results in down-regulation of this pathway. In this study, we have discovered that Nbp2p orthologues exist in all Ascomycete and Basidiomycete fungal genomes and that all possess an SH3 domain and a conserved novel Ptc1p binding motif. The ubiquitous occurrence of these two features, which we have shown are both critical for Nbp2p function in Saccharomyces cerevisiae, implies that a conserved role of Nbp2p in all of these fungal species is the targeting of Ptc1p to proteins recognized by the SH3 domain. We also show that in a manner analogous to its role in the high osmolarity glycerol pathway, Nbp2p functions in the down-regulation of the cell wall integrity pathway through SH3 domain-mediated interaction with Bck1p, a component kinase of this pathway...Continue Reading

References

Jan 11, 2001·Protein Science : a Publication of the Protein Society·S M Larson, A R Davidson
Jul 14, 2001·American Journal of Health-system Pharmacy : AJHP : Official Journal of the American Society of Health-System Pharmacists·A Hoang
Dec 20, 2003·The EMBO Journal·James Mapes, Irene M Ota
Jun 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hildur V ColotJay C Dunlap
Sep 16, 2006·The EMBO Journal·Yunrui DuSusan Ferro-Novick
May 10, 2007·Mycologia·John W Taylor, Mary L Berbee
Feb 17, 2009·Eukaryotic Cell·Clinton R Bartholomew, Christopher F J Hardy
Jan 23, 2010·Science·Michael CostanzoCharles Boone
Apr 20, 2010·Nature·Mohan K MalleshaiahStephen W Michnick
May 22, 2010·Science·Ashton BreitkreutzMike Tyers
Apr 15, 2011·The Journal of Biological Chemistry·Maryna GorelikAlan R Davidson

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Citations

Feb 20, 2014·Biochemical and Biophysical Research Communications·Nils Johnsson
Jun 10, 2017·International Journal of Molecular Sciences·Ewa ZatorskaSabine Strahl
Apr 19, 2015·Molecular Systems Biology·Humberto MartinNevan J Krogan
May 21, 2020·Molecular & Cellular Proteomics : MCP·Cynthia CheliusMark R Marten
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Cynthia CheliusMark R Marten
Feb 25, 2021·Molecular Biology of the Cell·Veneta GerganovaSophie G Martin

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