Yeast cells as microcapsules. Analytical tools and process variables in the encapsulation of hydrophobes in S. cerevisiae

Applied Microbiology and Biotechnology
Federica CiamponiNicola Tirelli

Abstract

Yeast cells can be used as biocompatible and biodegradable containers for the microencapsulation of a variety of actives. Despite the wide application of this process, e.g. in the food industry, mechanism and controlling factors are yet poorly known. In this study we have studied kinetics and mechanistic aspects of the spontaneous internalization of terpenes (as model hydrophobic compounds) in Saccharomyces cerevisiae, quantifying their encapsulation through HPLC analysis and fluorescent staining of lipidic bodies with Nile Red, while in parallel monitoring cell viability. Our results showed that this encapsulation process is essentially a phenomenon of passive diffusion with negligible relevance of active transport. Further, our evidence shows that the major determinant of the encapsulation kinetics is the solubility of the hydrophobe in the cell wall, which is inversely related to partition coefficient (log P).

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Citations

Jul 4, 2013·Applied Microbiology and Biotechnology·Bao Ngoc Pham-HoangYves Waché
Mar 17, 2016·Applied Microbiology and Biotechnology·Antonio Anchieta de CâmaraMárcia Regina da Silva Pedrini
Dec 10, 2013·Applied Microbiology and Biotechnology·Marcia Regina da Silva PedriniPatrick Gervais
Nov 5, 2016·Colloids and Surfaces. B, Biointerfaces·Bao Ngoc Pham-HoangYves Waché
Mar 10, 2015·Applied and Environmental Microbiology·Timothy C R BrennanLars K Nielsen
Nov 7, 2019·Applied Microbiology and Biotechnology·Fábio Gonçalves Macêdo de MedeirosMárcia Regina da Silva Pedrini
Jan 9, 2021·Biotechnology for Biofuels·Hannah G BlitzblauA Joe Shaw
Aug 28, 2020·Food Research International·Gabriela Vollet MarsonMiriam Dupas Hubinger
Feb 27, 2021·Biotechnology Progress·Elahe DadkhodazadeEhsan Moghaddas Kia
Feb 7, 2019·Food Chemistry·Stephen Young, Nitin Nitin
Jun 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Giulia Coradello, Nicola Tirelli
Apr 11, 2019·Journal of Agricultural and Food Chemistry·Eleni KavetsouAnastasia Detsi
Sep 25, 2021·International Journal of Pharmaceutics·Fang DouNitin Nitin

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